国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2014年
7期
1197-1202
,共6页
王丽波%李海%巫宇舟%王懿%伍凯强
王麗波%李海%巫宇舟%王懿%伍凱彊
왕려파%리해%무우주%왕의%오개강
翼状胬肉%角膜内皮生长因子%CD34%Ki-67%p21%免疫组织化学
翼狀胬肉%角膜內皮生長因子%CD34%Ki-67%p21%免疫組織化學
익상노육%각막내피생장인자%CD34%Ki-67%p21%면역조직화학
pterygium%VEGF%CD34%Ki - 67%p21%immunohistochemistry
目的：研究VEGF、CD34、Ki-67和p21在翼状胬肉中的表达,探讨它们与胬肉临床病理特征的关系,进一步探讨其发病机制。<br> 方法：采用免疫组织化学SP法,检测VEGF、CD34、Ki-67和p21在62例翼状胬肉和对照组20例结膜组织中的表达情况,并分析其与胬肉临床病理特征的关系。<br> 结果：(1)62例翼状胬肉组织中,VEGF、CD34、Ki-67和p21的阳性表达率分别为74.2%(46/62),77.4%(48/62),66.1%(41/62),40.3%(25/62),与对照组相比,差异均有统计学意义(P<0.05)；(2)在62例翼状胬肉组织中, VEGF、CD34的表达与临床分期及临床类型关系密切( P<0.05),与患者性别、年龄、职业等无关(均为P>0.05)；Ki-67的表达与临床分期关系密切(P<0.05),与其它临床病理指标无关(均为P>0.05)； p21的表达与临床分期、胬肉性质关系密切(P<0.05),与其它临床病理指标无关(均为P>0.05)；(3) VEGF与 Ki-67的表达呈正相关( r=0.279, P<0.05)；VEGF与 CD34的表达呈正相关(r=0.299,P<0.05)；VEGF与p21表达呈负相关(r=-0.267,P<0.05)；Ki-67与CD34的表达无相关性(r=0.021,P>0.05)； Ki-67与p21的表达无相关性(r=-0.176,P>0.05)；CD34与p21的表达无相关性(r=-0.185,P>0.05)。<br> 结论：(1) VEGF、CD34和Ki-67在翼状胬肉中高表达, p21在翼状胬肉中低表达,提示它们可能参与了翼状胬肉的发生发展过程；(2) VEGF、CD34在翼状胬肉中的阳性表达率随临床分期、临床类型的提高而上升,Ki-67在翼状胬肉中的阳性表达率随临床分期的提高而上升,p21在翼状胬肉的阳性表达率随临床分期、临床类型进展而降低,提示它们可能在翼状胬肉的发生发展及复发过程中发生了作用；(3) VEGF 与 Ki-67的表达呈正相关, VEGF与CD34的表达呈正相关, VEGF与p21的表达呈负相关,提示两两之间在翼状胬肉发生发展中具有协同作用。
目的：研究VEGF、CD34、Ki-67和p21在翼狀胬肉中的錶達,探討它們與胬肉臨床病理特徵的關繫,進一步探討其髮病機製。<br> 方法：採用免疫組織化學SP法,檢測VEGF、CD34、Ki-67和p21在62例翼狀胬肉和對照組20例結膜組織中的錶達情況,併分析其與胬肉臨床病理特徵的關繫。<br> 結果：(1)62例翼狀胬肉組織中,VEGF、CD34、Ki-67和p21的暘性錶達率分彆為74.2%(46/62),77.4%(48/62),66.1%(41/62),40.3%(25/62),與對照組相比,差異均有統計學意義(P<0.05)；(2)在62例翼狀胬肉組織中, VEGF、CD34的錶達與臨床分期及臨床類型關繫密切( P<0.05),與患者性彆、年齡、職業等無關(均為P>0.05)；Ki-67的錶達與臨床分期關繫密切(P<0.05),與其它臨床病理指標無關(均為P>0.05)； p21的錶達與臨床分期、胬肉性質關繫密切(P<0.05),與其它臨床病理指標無關(均為P>0.05)；(3) VEGF與 Ki-67的錶達呈正相關( r=0.279, P<0.05)；VEGF與 CD34的錶達呈正相關(r=0.299,P<0.05)；VEGF與p21錶達呈負相關(r=-0.267,P<0.05)；Ki-67與CD34的錶達無相關性(r=0.021,P>0.05)； Ki-67與p21的錶達無相關性(r=-0.176,P>0.05)；CD34與p21的錶達無相關性(r=-0.185,P>0.05)。<br> 結論：(1) VEGF、CD34和Ki-67在翼狀胬肉中高錶達, p21在翼狀胬肉中低錶達,提示它們可能參與瞭翼狀胬肉的髮生髮展過程；(2) VEGF、CD34在翼狀胬肉中的暘性錶達率隨臨床分期、臨床類型的提高而上升,Ki-67在翼狀胬肉中的暘性錶達率隨臨床分期的提高而上升,p21在翼狀胬肉的暘性錶達率隨臨床分期、臨床類型進展而降低,提示它們可能在翼狀胬肉的髮生髮展及複髮過程中髮生瞭作用；(3) VEGF 與 Ki-67的錶達呈正相關, VEGF與CD34的錶達呈正相關, VEGF與p21的錶達呈負相關,提示兩兩之間在翼狀胬肉髮生髮展中具有協同作用。
목적：연구VEGF、CD34、Ki-67화p21재익상노육중적표체,탐토타문여노육림상병리특정적관계,진일보탐토기발병궤제。<br> 방법：채용면역조직화학SP법,검측VEGF、CD34、Ki-67화p21재62례익상노육화대조조20례결막조직중적표체정황,병분석기여노육림상병리특정적관계。<br> 결과：(1)62례익상노육조직중,VEGF、CD34、Ki-67화p21적양성표체솔분별위74.2%(46/62),77.4%(48/62),66.1%(41/62),40.3%(25/62),여대조조상비,차이균유통계학의의(P<0.05)；(2)재62례익상노육조직중, VEGF、CD34적표체여림상분기급림상류형관계밀절( P<0.05),여환자성별、년령、직업등무관(균위P>0.05)；Ki-67적표체여림상분기관계밀절(P<0.05),여기타림상병리지표무관(균위P>0.05)； p21적표체여림상분기、노육성질관계밀절(P<0.05),여기타림상병리지표무관(균위P>0.05)；(3) VEGF여 Ki-67적표체정정상관( r=0.279, P<0.05)；VEGF여 CD34적표체정정상관(r=0.299,P<0.05)；VEGF여p21표체정부상관(r=-0.267,P<0.05)；Ki-67여CD34적표체무상관성(r=0.021,P>0.05)； Ki-67여p21적표체무상관성(r=-0.176,P>0.05)；CD34여p21적표체무상관성(r=-0.185,P>0.05)。<br> 결론：(1) VEGF、CD34화Ki-67재익상노육중고표체, p21재익상노육중저표체,제시타문가능삼여료익상노육적발생발전과정；(2) VEGF、CD34재익상노육중적양성표체솔수림상분기、림상류형적제고이상승,Ki-67재익상노육중적양성표체솔수림상분기적제고이상승,p21재익상노육적양성표체솔수림상분기、림상류형진전이강저,제시타문가능재익상노육적발생발전급복발과정중발생료작용；(3) VEGF 여 Ki-67적표체정정상관, VEGF여CD34적표체정정상관, VEGF여p21적표체정부상관,제시량량지간재익상노육발생발전중구유협동작용。
AlM:To investigate the expression of VEGF, CD34, Ki-67 and p21 in pterygium as well as the correlation between their expression and clinical pathological characteristics;explore its pathogenesis. <br> METHODS: lmmunohistochemical S - P staining method was adopted in detecting the expression of VEGF, CD34, Ki-67 and p21 in 62 cases of pterygia and 20 cases of normal conjunctival tissues. Relationship between these markers and clinical pathological characteristics was analyzed. <br> RESULTS:(1) The positive expression of VEGF, CD34, Ki-67 and p21 in 62 cases of pterygia was 74. 2% (46/62), 77. 4% ( 48/62 ), 66. 1% ( 41/62 ) and 40. 3% ( 25/62 ) respectively. The differences were statistically significant compared with normal conjunctival tissues (P<0. 05). (2) The expression of VEGF and CD34 in 62 cases of pterygia was correlated with clinical types and stages (P<0. 05), and was not associated with sex, age and occupation ( P>0. 05 ); the expression of Ki-67 was correlated with clinical stages (P<0. 05), and was not associated with other clinical pathological characteristics ( P>0. 05 ); the expression of p21 was correlated with clinical stages and pterygium characters (P<0. 05), and was not associated with other clinical pathological characteristics ( P> 0. 05 ). ( 3 ) Spearman correlation showed that there was a positive correlation between VEGF and Ki - 67 ( r = 0. 279, P < 0. 05 ), a positive correlation between VEGF and CD34 (r=0. 299, P<0. 05), a negative correlation between VEGF and p21 (r=-0. 267, P<0. 05 ); it also showed that there was no correlation between any two of CD34, Ki-67 and p21 (P>0. 05). <br> CONCLUSlON: ( 1 ) Overexpression of VEGF, Ki-67, CD34 and low expression of p21 suggest that these markers are concerned with the development and progression of pterygium. ( 2 ) Expression of VEGF and CD34 increases along with the increase of clinical types and stages, expression of Ki-67 increases along with the increase of clinical stages, and expression of p21 decreases along with the improvement of clinical types or stages; they suggest that these markers may play important roles in the development and recurrence of pterygium. ( 3 ) There is positive correlation between VEGF and Ki-67, VEGF and CD34 as well as negative correlation between VEGF and p21. They suggest that there may be synergistic action between two factors during the development and progression of pterygium.
