目的：初步研究恒温条件下兔角膜热烧伤后继发的角膜新生血管( CNV)快速制模的合适条件。<br> 方法：新西兰大白兔45只,随机分为( A,B,C,D,E)五组恒温烧灼器诱导实验性CNV模型,除E组为5只,其他组均为10只。 A组：100℃, B组：200℃, C组：300℃, D组：400℃,E组：空白对照。以左眼为实验眼,比较建模后第4,7,14,30 d在裂隙灯显微镜下观察各组角膜新生血管的生长情况并计算角膜新生血管生长面积。采用SPSS 19.0统计软件包对数据进行分析,计算资料用x-±s表示,各时间点各自新生血管面积的比较采用重复测量数据的方差分析；显著性标准为α=0.05。<br> 结果：制模后第4,7,14,30d,A组角膜热烧伤后无明显新生血管生长,仅少量留有角膜云翳(n=2)。 B组新生血管面积分别为：9.16±1.45,37.73±5.49,62.44±7.54,40.28±7.39mm2；C组新生血管面积分别为：11.45±1.04,44.51±4.64,66.13±4.13,43.04±2.33mm2；D组新生血管面积分别为：13.23±0.86,47.26±4.59,67.57±4.56,45.59±4.44mm2；D组部分角膜局部出现焦化(n=4),3d内出现穿孔( n=6)。 E组未见新生血管生长。各时间点的CNV面积比较,差异有显著性(P<0.05),B,C,D分组之间有统计学差异(P<0.05)。<br> 结论：角膜热烧伤4~7d时,制模温度越高,新生血管生长越快,面积越大；14~30 d 新生血管面积无明显差别,但400℃角膜制模失败率高,200℃~300℃制作的兔角膜新生血管模型均一性及重复性高。
目的：初步研究恆溫條件下兔角膜熱燒傷後繼髮的角膜新生血管( CNV)快速製模的閤適條件。<br> 方法：新西蘭大白兔45隻,隨機分為( A,B,C,D,E)五組恆溫燒灼器誘導實驗性CNV模型,除E組為5隻,其他組均為10隻。 A組：100℃, B組：200℃, C組：300℃, D組：400℃,E組：空白對照。以左眼為實驗眼,比較建模後第4,7,14,30 d在裂隙燈顯微鏡下觀察各組角膜新生血管的生長情況併計算角膜新生血管生長麵積。採用SPSS 19.0統計軟件包對數據進行分析,計算資料用x-±s錶示,各時間點各自新生血管麵積的比較採用重複測量數據的方差分析；顯著性標準為α=0.05。<br> 結果：製模後第4,7,14,30d,A組角膜熱燒傷後無明顯新生血管生長,僅少量留有角膜雲翳(n=2)。 B組新生血管麵積分彆為：9.16±1.45,37.73±5.49,62.44±7.54,40.28±7.39mm2；C組新生血管麵積分彆為：11.45±1.04,44.51±4.64,66.13±4.13,43.04±2.33mm2；D組新生血管麵積分彆為：13.23±0.86,47.26±4.59,67.57±4.56,45.59±4.44mm2；D組部分角膜跼部齣現焦化(n=4),3d內齣現穿孔( n=6)。 E組未見新生血管生長。各時間點的CNV麵積比較,差異有顯著性(P<0.05),B,C,D分組之間有統計學差異(P<0.05)。<br> 結論：角膜熱燒傷4~7d時,製模溫度越高,新生血管生長越快,麵積越大；14~30 d 新生血管麵積無明顯差彆,但400℃角膜製模失敗率高,200℃~300℃製作的兔角膜新生血管模型均一性及重複性高。
목적：초보연구항온조건하토각막열소상후계발적각막신생혈관( CNV)쾌속제모적합괄조건。<br> 방법：신서란대백토45지,수궤분위( A,B,C,D,E)오조항온소작기유도실험성CNV모형,제E조위5지,기타조균위10지。 A조：100℃, B조：200℃, C조：300℃, D조：400℃,E조：공백대조。이좌안위실험안,비교건모후제4,7,14,30 d재렬극등현미경하관찰각조각막신생혈관적생장정황병계산각막신생혈관생장면적。채용SPSS 19.0통계연건포대수거진행분석,계산자료용x-±s표시,각시간점각자신생혈관면적적비교채용중복측량수거적방차분석；현저성표준위α=0.05。<br> 결과：제모후제4,7,14,30d,A조각막열소상후무명현신생혈관생장,부소량류유각막운예(n=2)。 B조신생혈관면적분별위：9.16±1.45,37.73±5.49,62.44±7.54,40.28±7.39mm2；C조신생혈관면적분별위：11.45±1.04,44.51±4.64,66.13±4.13,43.04±2.33mm2；D조신생혈관면적분별위：13.23±0.86,47.26±4.59,67.57±4.56,45.59±4.44mm2；D조부분각막국부출현초화(n=4),3d내출현천공( n=6)。 E조미견신생혈관생장。각시간점적CNV면적비교,차이유현저성(P<0.05),B,C,D분조지간유통계학차이(P<0.05)。<br> 결론：각막열소상4~7d시,제모온도월고,신생혈관생장월쾌,면적월대；14~30 d 신생혈관면적무명현차별,단400℃각막제모실패솔고,200℃~300℃제작적토각막신생혈관모형균일성급중복성고。
AlM:To explore the suitable conditions in rapid model of corneal neovascularization ( CNV ) after thermal burn under different constant temperature in rabbit. <br> METHODS: Total 45 New Zealand white rabbits were divided randomly into five groups ( A, B, C, D, E ) . A groups:100℃ ( n = 10 ) , B groups: 200℃ ( n = 10 ) , C groups:300℃ ( n=10 ) , D groups: 400℃ ( n=10 ) , and E groups:control group ( n=5 ) . All left eyes of rabbits in A,B,C,D groups were induced corneal neovascularization by constant temperature burning device. The growth of CNV was observed by slit lamp microscope and the area of CNV were recorded on 4 th , 7 th , 14 th , 30 th days postoperatively. SPSS 19. 0 statistical package was used for data analysis, and the data was recorded by mean ± standard deviation. Comparison by analysis of variance was made by repeated measures in the area of neovascularization at each time point in groups. Statistical tests were considered significantly when P values were less than 0. 05. <br> RESULTS:On postoperative 4 th , 7 th , 14 th , 30 th days: no neovascularization was found after corneal thermal burn in A group, but only a few nebula left (n=2);the area of CNV were (9.16±1.45)mm2, (37.73±5.49)mm2, (62.44± 7. 54 ) mm2 , ( 40. 28 ± 7. 39 ) mm2 in B group respectively;and (11.45±1.04)mm2, (44.51±4.64)mm2, (66.13±4.13)mm2, (43.04±2.33)mm2 in C group respectively; and (13.23± 0.86)mm2,(47.26±4.59)mm2,(67.57±4.56)mm2,(45.59± 4. 44 ) mm2 in D group respectively, and part corneal carbide ( n = 4 ) was observed as well as corneal perforation ( n= 6 ) were found on 3d in D group. No neovascularization was found in normal control group. Comparison of the areas of CNV at each time point between groups was statistically different, P < 0. 05. Statistical differences were found among B, C, D groups, P<0. 05. <br> COCLUSlON:ln 4 to 7d, the higher the temperature is, the more the neovascularization area of CNV are. lt has no significant difference in 14 to 30d. But corneal carbide and corneal perforation are often found in 400℃ group, so its modeling failure rate is high. lt is between 200℃ and 300℃ that repeatability and uniformity of the corneal neovascularization model of rabbit are superior.
