CAJ | 학술논문

选择生长发育正常的绿壳蛋鸡15000只，随机分成笼养高密度组、笼养低密度组和散养组，每组又分为对照组和实验组，2500只/组。对照组饲喂正常日粮，实验组饲喂添加0.1%的牛磺酸日粮。在实验期第90天，每组随机选取10只采集血液和肾脏，进行肾脏形态和相关生化指标检测。结果显示：饲养方式和日粮中添加0.1%牛磺酸对血清中SCr和UA含量没有显著影响（P＞0.05）；笼养蛋鸡血清BUN含量无论在高密度组还是低密度组均显著高于散养组（P＜0.05）。iNOs活性、TNF-α、IFN-γ、IL-4和IL-10等和炎症免疫相关的细胞因子水平在各饲养模式及处理间均无显著差异。饲养方式对肾脏组织中MDA含量、SOD和T-AOC活性无显著影响。散养组肾脏T-AOC活性显著高于笼养高密度组（P＜0.05）；日粮中添加0.1%牛磺酸可以显著升高散养组和笼养高密度组肾脏T-AOC活性（P＜0.05）。结果表明，散养、降低饲养密度和日粮中添加牛磺酸可以提高肾脏组织抗氧化水平，调控机体的代谢，改善饲养应激造成的肾脏组织损伤，进而提高蛋鸡的健康水平。
선택생장발육정상적록각단계15000지，수궤분성롱양고밀도조、롱양저밀도조화산양조，매조우분위대조조화실험조，2500지/조。대조조사위정상일량，실험조사위첨가0.1%적우광산일량。재실험기제90천，매조수궤선취10지채집혈액화신장，진행신장형태화상관생화지표검측。결과현시：사양방식화일량중첨가0.1%우광산대혈청중SCr화UA함량몰유현저영향（P＞0.05）；롱양단계혈청BUN함량무론재고밀도조환시저밀도조균현저고우산양조（P＜0.05）。iNOs활성、TNF-α、IFN-γ、IL-4화IL-10등화염증면역상관적세포인자수평재각사양모식급처리간균무현저차이。사양방식대신장조직중MDA함량、SOD화T-AOC활성무현저영향。산양조신장T-AOC활성현저고우롱양고밀도조（P＜0.05）；일량중첨가0.1%우광산가이현저승고산양조화롱양고밀도조신장T-AOC활성（P＜0.05）。결과표명，산양、강저사양밀도화일량중첨가우광산가이제고신장조직항양화수평，조공궤체적대사，개선사양응격조성적신장조직손상，진이제고단계적건강수평。
15 000 green-shell laying hens(148 day-old)were randomly assigned into a free range group, and 2 caged groups with low-density and high-density housing, respectively. Each group was further divided into the control(C)and taurine treatment(T) groups. All the test subjects were fed the same basic diet except that the T groups was supplemented with 0.1% taurine. After 90 days, serum, kidneys were aseptically collected. The results show that there was no significant change of serum concentrations of SCr and UA between different groups(P>0.05). Compared with free range group, serum BUN concentrations were higher both in high-density group and low-density group(P<0.05). No significant difference was observed on iNOs activities and TNF-α, IFN-γ, IL-4, IL-10 concentrations in different groups. There was no significant change of MDA content, SOD and T-AOC activity in kidney tissue under different rearing patterns(P>0.05). Compared with high-density group, the T-AOC levels in free range group increased obviously(P<0.05). The T-AOC levels of free range group and caged groups with high-density housing with 0.1% taurine increased significantly(P<0.05). The data indicate that free range, reduced housing density and taurine could influence anti-oxidation level, regulate metabolism and protect the normal function of the kidney and thus enhance the health of laying hens.