CAJ | 학술논문

目的：研究一种纳米金增强心肌肌钙蛋白I（cTnI）金标免疫层析试验灵敏度的试验方法。方法以血清cTnI金标免疫层析试验为基础，以改性溶液做预处理，盐酸羟胺还原氯金酸制备纳米金颗粒，使金标抗原抗体反应复合物的颗粒增大，金标处颜色加深，显色信号增强，提高检测灵敏度，并以此做批内和日间重复性检测。结果盐酸羟胺还原氯金酸增敏cTnI金标免疫层析试验，较增敏前检测灵敏度提高16倍，在10～15 min内可完成，批内和日间重复性较好，试验结果快速、稳定、可靠。结论盐酸羟胺还原氯金酸制备纳米金颗粒在一定改性溶液环境下，对人血清cTnI金标免疫层析试验有一定增敏效果，重复性较好，结果稳定可靠，可为适时、快速、灵敏的现场检测方法提供一定技术支持。
목적：연구일충납미금증강심기기개단백I（cTnI）금표면역층석시험령민도적시험방법。방법이혈청cTnI금표면역층석시험위기출，이개성용액주예처리，염산간알환원록금산제비납미금과립，사금표항원항체반응복합물적과립증대，금표처안색가심，현색신호증강，제고검측령민도，병이차주비내화일간중복성검측。결과염산간알환원록금산증민cTnI금표면역층석시험，교증민전검측령민도제고16배，재10～15 min내가완성，비내화일간중복성교호，시험결과쾌속、은정、가고。결론염산간알환원록금산제비납미금과립재일정개성용액배경하，대인혈청cTnI금표면역층석시험유일정증민효과，중복성교호，결과은정가고，가위괄시、쾌속、령민적현장검측방법제공일정기술지지。
Objective To study a new assay for enhancing the sensitivity of cTnI Glod Immunochromatographic assay quickly by Au nanoparticles .Meth ods The serum cTnI was qualitatively detected by glod immunochromatographic assay ( GICA) and on this basis,the reaction bands of GICA were treated by modification solution ,and bound the colloidal gold prepared by NH 2 OH· HCl reduc-ing HAuCl4 ,then the diameter of antigen-antibody complex labeled by the colloidal gold was expanded and its color became darker and the signal turned stronger and so the sensitivity was enhanced .At the same time,the intra and between-day repeatability of this method was analyzed .Results The sensitivity was increased 16 times and had better repeatability compared with that of the initial test in this method.Conclusion Colloidal gold prepared by NH2OH· HCl reducing HAuCl4 can be applied as sensitizing reagent under certain circumstances and can enhance the sensitivity of cTnI GICA quickly with stable and reliable result .It may provide a new highly sensi-tive technical support to the poin-of-care testing .