CAJ | 학술논문

目的：通过研究神经轴突导向因子Slit3及Robo4受体在大鼠正常角膜与新生血管化角膜中的差异性表达,探讨其在角膜新生血管( corneal neovascularization,CNV)形成中的作用。 　　方法：采用角膜微囊袋法建立以碱性成纤维细胞生长因子( bFGF)诱导的大鼠CNV模型,采用眼前段照相,免疫组化染色、彩色图像分析系统分别计算1,4,7,10,14d共5个时间点的CNV面积和Slit3, Robo4的平均光密度值。结果：Slit3及Robo4在新生血管化角膜中表达增加,在第7 d达到最高,且二者的表达水平与 CNV 的面积除第1 d外,在其他各时间点均呈正相关(r=0.84~0.91,P<0.05)。结论：Slit3/Robo4与CNV形成明显相关,其可能成为抑制CNV的重要靶点。
목적：통과연구신경축돌도향인자Slit3급Robo4수체재대서정상각막여신생혈관화각막중적차이성표체,탐토기재각막신생혈관( corneal neovascularization,CNV)형성중적작용。 　　방법：채용각막미낭대법건립이감성성섬유세포생장인자( bFGF)유도적대서CNV모형,채용안전단조상,면역조화염색、채색도상분석계통분별계산1,4,7,10,14d공5개시간점적CNV면적화Slit3, Robo4적평균광밀도치。결과：Slit3급Robo4재신생혈관화각막중표체증가,재제7 d체도최고,차이자적표체수평여 CNV 적면적제제1 d외,재기타각시간점균정정상관(r=0.84~0.91,P<0.05)。결론：Slit3/Robo4여CNV형성명현상관,기가능성위억제CNV적중요파점。
AlM: To explore the roles of neuronal axon-guidance molecules Slit3 and Robo4 receptor in corneal neovascularization ( CNV ) by study their expression in neovascularized cornea of rats. METHODS: CNV models were established by implantation pellets containing basic fibroblast growth factor ( bFGF ) into corneal stroma. CNV models were measured by biomicroscopy photography. lmmunohistochemical staining and imaging analysis system were used to detect the expression of Slit3 and Robo4 in the models after 1, 4, 7, 10 and 14d. RESULTS:The area of CNV and the expression of Slit3, Robo4 were increased in CNV models compared to that in normal cornea and reached highest level on 7d. And the expression level of Slit3 and Robo4 were significantly correlated with the size of CNV on every time point except 1d (r=0. 84-0. 91, all P<0. 05). CONCLUSlON: The expression of Slit3 and Robo4 may be related to the CNV development. They are potential therapeutical target for CNV.