CAJ | 학술논문

目的：探讨随机扩增多态性DNA（RAPD）分析法在在幽门螺杆菌（HP）菌株鉴定中的应用价值。方法选取该院65例HP感染的慢性胃炎或消化性溃疡患者，获取胃窦、胃体部黏膜进行 HP培养，提取及纯化HP DNA后进行PCR反应，并采用RAPD分析HP基因指纹图谱；以同一HP菌株验证RAPD可重复性；65例患者均进行6个月以上的 HP治疗，治疗后4～6周评估HP治愈情况；对复发病例再次进行RAPD分析，观察RAPD指纹图谱变化。结果65株菌株PAPD指纹图谱各不相同，但同一菌株传4代后，原代株与传代株进行 PCR扩增，RADP指纹图谱完全一致。6例HP治疗后复发，3例RAPD指纹图谱与治疗前基本一致；3例产生不同RAPD指纹图谱。结论 RAPD分析法适用于 HP基因多态性鉴定，是临床研究 HP感染的实用方法，可用于HP复发和再感染鉴定。
목적：탐토수궤확증다태성DNA（RAPD）분석법재재유문라간균（HP）균주감정중적응용개치。방법선취해원65례HP감염적만성위염혹소화성궤양환자，획취위두、위체부점막진행 HP배양，제취급순화HP DNA후진행PCR반응，병채용RAPD분석HP기인지문도보；이동일HP균주험증RAPD가중복성；65례환자균진행6개월이상적 HP치료，치료후4～6주평고HP치유정황；대복발병례재차진행RAPD분석，관찰RAPD지문도보변화。결과65주균주PAPD지문도보각불상동，단동일균주전4대후，원대주여전대주진행 PCR확증，RADP지문도보완전일치。6례HP치료후복발，3례RAPD지문도보여치료전기본일치；3례산생불동RAPD지문도보。결론 RAPD분석법괄용우 HP기인다태성감정，시림상연구 HP감염적실용방법，가용우HP복발화재감염감정。
Objective To investigate application value of random amplified polymorphic DNA analysis in the identifi-cation of HP .Methods 65 patients with peptic ulcer or chronic gastritis ,which confirm as HP infection ,get orget gastric body mucosa carried HP culture ,after HP DNA extraction and purification ,and analysis using HP RAPD genetic finger-printing ;strains of the same HP RAPD verify repeatability ,65 patients underwent six months or more HP eradication thera-py ,assess the situation Hp eradication 4-6 weeks after treatment ;recurrence again on RAPD analysis ,the changes of RAPD fingerprint were observed .Results 65 strains PAPD fingerprints are different ,but RADP fingerprints of the original genera-tion plants and extend the plant after 4 passages is same .Three cases is consistant in RAPD fingerprinting befor and after treatment ,3 cases have different RAPD fingerprints in six cases of relapse after HP eradication .Conclusion RAPD analysis of genetic polymorphisms identified for HP ,is a useful tool for clinical studies ,it can be used to identify HP relapse and rein-fection .