中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
2期
183-185
,共3页
谢永刚%李爱芝%姜秀良%马加海
謝永剛%李愛芝%薑秀良%馬加海
사영강%리애지%강수량%마가해
神经节苷酯类%脊髓损伤%疼痛%注射,脊髓
神經節苷酯類%脊髓損傷%疼痛%註射,脊髓
신경절감지류%척수손상%동통%주사,척수
Gangliosides%Spinal cord injuries%Pain%Injections,spinal
目的 评价鞘内注射神经节苷酯GM-1对大鼠脊髓损伤后慢性中枢性痛的影响.方法 雄性成年SD大鼠,体重220 ～ 250 g,进行鞘内置管取鞘内置管成功的大鼠32只,采用随机数字表法,将大鼠分成4组(n=8):对照组(C组)、慢性中枢性痛组(CCP组)、生理盐水组(N组)和神经节苷酯GM-1组(GM 组).CCP 组、N组和GM组采用改良Allen法建立大鼠脊髓损伤后慢性中枢性痛模型.造模成功后第14天,GM组鞘内注射神经节苷酯GM-1生理盐水稀释液20 mg/kg,1次/d1,连续5d;N组鞘内注射生理盐水10 μl. 于给药结束后4、8、16、24和48 h时测定机械痛阈和热痛阈.于给药结束后7d处死大鼠,取L41节段脊髓组织,采用免疫组化法测定含1亚基的NMDA受体(NR1)的表达水平.结果 与C组比较,CCP组和N组机械痛阈和热痛阈降低,脊髓NR1表达上调(P＜0.01),GM组机械痛阈、热痛阈和脊髓NR1表达差异无统计学意义(P＞0.05);与CCP组比较,N组机械痛阈、热痛阈和脊髓NR1表达差异无统计学意义(P＞0.05),GM组机械痛阈和热痛阈升高,脊髓NR1表达下调(P＜0.05).结论 鞘内注射神经节苷酯GM-1可减轻大鼠脊髓损伤后慢性中枢性痛,其机制与抑制脊髓NR1的表达有关.
目的 評價鞘內註射神經節苷酯GM-1對大鼠脊髓損傷後慢性中樞性痛的影響.方法 雄性成年SD大鼠,體重220 ～ 250 g,進行鞘內置管取鞘內置管成功的大鼠32隻,採用隨機數字錶法,將大鼠分成4組(n=8):對照組(C組)、慢性中樞性痛組(CCP組)、生理鹽水組(N組)和神經節苷酯GM-1組(GM 組).CCP 組、N組和GM組採用改良Allen法建立大鼠脊髓損傷後慢性中樞性痛模型.造模成功後第14天,GM組鞘內註射神經節苷酯GM-1生理鹽水稀釋液20 mg/kg,1次/d1,連續5d;N組鞘內註射生理鹽水10 μl. 于給藥結束後4、8、16、24和48 h時測定機械痛閾和熱痛閾.于給藥結束後7d處死大鼠,取L41節段脊髓組織,採用免疫組化法測定含1亞基的NMDA受體(NR1)的錶達水平.結果 與C組比較,CCP組和N組機械痛閾和熱痛閾降低,脊髓NR1錶達上調(P＜0.01),GM組機械痛閾、熱痛閾和脊髓NR1錶達差異無統計學意義(P＞0.05);與CCP組比較,N組機械痛閾、熱痛閾和脊髓NR1錶達差異無統計學意義(P＞0.05),GM組機械痛閾和熱痛閾升高,脊髓NR1錶達下調(P＜0.05).結論 鞘內註射神經節苷酯GM-1可減輕大鼠脊髓損傷後慢性中樞性痛,其機製與抑製脊髓NR1的錶達有關.
목적 평개초내주사신경절감지GM-1대대서척수손상후만성중추성통적영향.방법 웅성성년SD대서,체중220 ～ 250 g,진행초내치관취초내치관성공적대서32지,채용수궤수자표법,장대서분성4조(n=8):대조조(C조)、만성중추성통조(CCP조)、생리염수조(N조)화신경절감지GM-1조(GM 조).CCP 조、N조화GM조채용개량Allen법건립대서척수손상후만성중추성통모형.조모성공후제14천,GM조초내주사신경절감지GM-1생리염수희석액20 mg/kg,1차/d1,련속5d;N조초내주사생리염수10 μl. 우급약결속후4、8、16、24화48 h시측정궤계통역화열통역.우급약결속후7d처사대서,취L41절단척수조직,채용면역조화법측정함1아기적NMDA수체(NR1)적표체수평.결과 여C조비교,CCP조화N조궤계통역화열통역강저,척수NR1표체상조(P＜0.01),GM조궤계통역、열통역화척수NR1표체차이무통계학의의(P＞0.05);여CCP조비교,N조궤계통역、열통역화척수NR1표체차이무통계학의의(P＞0.05),GM조궤계통역화열통역승고,척수NR1표체하조(P＜0.05).결론 초내주사신경절감지GM-1가감경대서척수손상후만성중추성통,기궤제여억제척수NR1적표체유관.
Objective To evaluate the effect of intrathecal ganglioside GM-1 on chronic central pain (CCP) following spinal cord injury in rats.Methods Thirty-two adult male Sprague-Dawley rats,weighing 220-250 g,in which intrathecal catheters were successfully implanted,were randomly divided into 4 groups (n =8 each) using a random number table:control group (group C); group CCP; normal saline group (group N) and ganglioside GM-1 group (group GM).CCP was induced according to modified Allen method in CCP,N and GM groups.In group GM,ganglioside GM-1 20 mg/kg was injected intrathecally once a day,for 5 consecutive days,starting from 14th day after CCP,while the equal volume of nomal saline 10 μl was injected intrathecally in group N.The mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured at 4,8,16,24 and 48 h after the end of administration.The rats were then sacrificedat at 7 d after the end of adminmistration and L1 segment of the spinal cord was removed for determination of the expression of NMDA receptor 1 (NR1) by immuno-histochemistry.Results Compared with group C,MWT and TWL were significantly decreased,and NR1 expression was up-regulated in CCP and N groups (P ＜ 0.01),and no significant changes were found in MWT,TWL and NR1 expression in group GM (P ＞ 0.05).Compared with group CCP,no significant changes were found in MWT,TWL and NR1 expression in group N (P ＞ 0.05),and MWT and TWL were significantly increased,and NR1 expression was down-regulated in group GM (P ＜ 0.05).Conclusion Ganglioside GM-1 can alleviate CCP following spinal cord injury in rats and inhibition of expression of NR1 in the spinal cord may be involved in the mechanism.
