检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2014年
12期
1608-1609,1612
,共3页
蔡叶琴%黄黎%宋俊%孙彦
蔡葉琴%黃黎%宋俊%孫彥
채협금%황려%송준%손언
肺炎支原体%间接免疫荧光法%被动颗粒凝集试验
肺炎支原體%間接免疫熒光法%被動顆粒凝集試驗
폐염지원체%간접면역형광법%피동과립응집시험
pneumonia mycoplasma%indirect immunofluorescence method%passive particle agglutination test
目的:探讨3种不同方法在小儿肺炎支原体检测中的应用效果。方法选择深圳市南山区西丽人民医院收治的1800例呼吸道感染患儿为研究对象,分别用间接免疫荧光法、被动颗粒凝集法、培养法检测支原体感染,并比较3种检测方法的阳性率、灵敏度、特异性。结果培养法阳性率与间接免疫荧光法及被动颗粒凝集法相比,差异无统计学意义(P>0.05);间接免疫荧光法与被动颗粒凝集法阳性率差异也无统计学意义(P>0.05);间接免疫荧光法与被动颗粒凝集法、培养法灵敏度、特异性经统计学分析,差异无统计学意义(P>0.05)。结论临床医师需根据病原体检测的优缺点,选择合适的方法,综合各种检测结果分析诊断肺炎支原体感染。
目的:探討3種不同方法在小兒肺炎支原體檢測中的應用效果。方法選擇深圳市南山區西麗人民醫院收治的1800例呼吸道感染患兒為研究對象,分彆用間接免疫熒光法、被動顆粒凝集法、培養法檢測支原體感染,併比較3種檢測方法的暘性率、靈敏度、特異性。結果培養法暘性率與間接免疫熒光法及被動顆粒凝集法相比,差異無統計學意義(P>0.05);間接免疫熒光法與被動顆粒凝集法暘性率差異也無統計學意義(P>0.05);間接免疫熒光法與被動顆粒凝集法、培養法靈敏度、特異性經統計學分析,差異無統計學意義(P>0.05)。結論臨床醫師需根據病原體檢測的優缺點,選擇閤適的方法,綜閤各種檢測結果分析診斷肺炎支原體感染。
목적:탐토3충불동방법재소인폐염지원체검측중적응용효과。방법선택심수시남산구서려인민의원수치적1800례호흡도감염환인위연구대상,분별용간접면역형광법、피동과립응집법、배양법검측지원체감염,병비교3충검측방법적양성솔、령민도、특이성。결과배양법양성솔여간접면역형광법급피동과립응집법상비,차이무통계학의의(P>0.05);간접면역형광법여피동과립응집법양성솔차이야무통계학의의(P>0.05);간접면역형광법여피동과립응집법、배양법령민도、특이성경통계학분석,차이무통계학의의(P>0.05)。결론림상의사수근거병원체검측적우결점,선택합괄적방법,종합각충검측결과분석진단폐염지원체감염。
Objective To study the clinical effect of the three different methods in the infantile pneumonia my-coplasma detection .Methods 1 800 cases of children with respiratory tract infection were choosed as the research ob-ject .indirect immunofluorescence method ,passive particle agglutination and culture method were used to detect myco-plasma pneumonia .Positive rate ,sensitivity and specificity of three methods were observed .Results PCR positive rate was significantly higher than the indirect immunofluorescence method and passive particle agglutination method , the difference was statistically significant (P<0 .05) .Indirect immunofluorescence method with the method of passive particle agglutination positive rate difference has no statistical significance (P>0 .05) .Indirect immunofluorescence and passive particle agglutination method ,PCR method ,the sensitivity ,specificity ,after statistics analysis ,there was no significant difference(P>0 .05) .Conclusion Clinicians should be based on the advantages and disadvantages of pathogen detection ,choosing the appropriate method ,based on the analysis of all kinds of test results in the diagnosis of mycoplasma pneumoniae infection .