CAJ | 학술논문

目的：探讨趋化因子5（CCL5）、白细胞介素-8（IL-8）与一氧化氮（NO）在骨关节炎（OA）发病中的相关性，以了解CCL5在OA发生发展中的生物学作用。方法应用酶联免疫吸附试验（ELISA ）检测CCL5、IL-8及硝酸还原法测定NO在关节滑液中的含量，分析CCL5与IL-8、NO在膝骨关节炎关节滑液中的相关性，研究对象包括膝关节炎（KOA ）患者56例，健康者25例。结果实验组与健康对照组CCL5、IL-8和NO 3个指标比较，差异有统计学意义（P＜0．01），KOA患者中CCL5、IL-8和 NO 3个指标的表达和健康者比较，差异有统计学意义（P＜0．05）。CCL5、IL-8和NO含量进行Spearman相关分析，相关系数 r分别为0．912、0．885（P＜0．01），均呈正相关。结论 CCL5可能是炎性因子IL-8和NO的趋化因子，其可促进KOA的局部非感染性炎性形成。
목적：탐토추화인자5（CCL5）、백세포개소-8（IL-8）여일양화담（NO）재골관절염（OA）발병중적상관성，이료해CCL5재OA발생발전중적생물학작용。방법응용매련면역흡부시험（ELISA ）검측CCL5、IL-8급초산환원법측정NO재관절활액중적함량，분석CCL5여IL-8、NO재슬골관절염관절활액중적상관성，연구대상포괄슬관절염（KOA ）환자56례，건강자25례。결과실험조여건강대조조CCL5、IL-8화NO 3개지표비교，차이유통계학의의（P＜0．01），KOA환자중CCL5、IL-8화 NO 3개지표적표체화건강자비교，차이유통계학의의（P＜0．05）。CCL5、IL-8화NO함량진행Spearman상관분석，상관계수 r분별위0．912、0．885（P＜0．01），균정정상관。결론 CCL5가능시염성인자IL-8화NO적추화인자，기가촉진KOA적국부비감염성염성형성。
Objective Discussion the correlation of osteoarthritis and the lever of CCL 5 ,IL-8 and NO in the pathogenesis and understand the effects of OA CCL5 in pathogenesis .Methods The level of CCL5 ,IL-8 were detec-ted by enzyme-linked immunosorbent assay ,the NO level in synovial fluid were analyzed by nitrate reduction .The correlation of osteoarthritis and the lever of CCL 5 ,IL-8 and NO in the pathogenesis were discussed in 56 patients with knee osteoarthritis (KOA) ,25 cases of healthy subjects .Results The level of CCL5 ,IL-8 and NO in the experi-mental group was statistically significant ,compared with the control group (P<0 .01) ,Spearman correlation analysis was contented to CCL5、IL-8 and NO .The correlation coefficients was r=0 .912 ,0 .885(P<0 .01) ,respectively ,those were positively correlated .Conclusion CCL5 may be inflammatory cytokines IL-8 and NO chemokines ,which pro-mote local KOA formation of non infectious inflammation .