浙江农业学报
浙江農業學報
절강농업학보
ACTA AGRICULTURAE ZHEJIANGENSIS
2014年
2期
530-536
,共7页
陈宇%王渭霞%陈洋%傅强
陳宇%王渭霞%陳洋%傅彊
진우%왕위하%진양%부강
Arsenophonus%分子鉴定%宿主范围%生物学功能%基因组
Arsenophonus%分子鑒定%宿主範圍%生物學功能%基因組
Arsenophonus%분자감정%숙주범위%생물학공능%기인조
Arsenophonus%molecular identification%host distribution%biological function%genome
Arsenophonus隶属于变形菌门(Proteobacteria)γ亚类肠杆菌科(Enterobacteriaceae),是昆虫、蜘蛛等体内常见共生细菌,还见于甜菜、草莓等植物。该菌在宿主昆虫体内既能垂直传播也能水平传播,在宿主昆虫体内多呈弥散性分布,少数分布于特化的菌胞体。一般通过16S rDNA,23S rDNA的特异序列或基于fbaA, ftzK和yaeT 的多基因座系统进行分子鉴定。迄今为止,只有5种Arsenophonus属的细菌被鉴定,分别是Arsenophonus nasoniae,Arsenophonus arthropodicus,Arsenophonus triatominarum,Riesia pediculicola和Phlomobacter frageriae。 Arsenophonus对宿主昆虫有杀雄作用,还可能与宿主昆虫的免疫、营养及寄主适应有关。同时, Arsenophonus还会诱发草莓和甜菜上的病害。目前,A.nasoniae和R.pediculicola已经完成了基因组的测序工作。文章主要对Arsenophonus的研究进行了综述,并对其未来的研究方向作了展望。
Arsenophonus隸屬于變形菌門(Proteobacteria)γ亞類腸桿菌科(Enterobacteriaceae),是昆蟲、蜘蛛等體內常見共生細菌,還見于甜菜、草莓等植物。該菌在宿主昆蟲體內既能垂直傳播也能水平傳播,在宿主昆蟲體內多呈瀰散性分佈,少數分佈于特化的菌胞體。一般通過16S rDNA,23S rDNA的特異序列或基于fbaA, ftzK和yaeT 的多基因座繫統進行分子鑒定。迄今為止,隻有5種Arsenophonus屬的細菌被鑒定,分彆是Arsenophonus nasoniae,Arsenophonus arthropodicus,Arsenophonus triatominarum,Riesia pediculicola和Phlomobacter frageriae。 Arsenophonus對宿主昆蟲有殺雄作用,還可能與宿主昆蟲的免疫、營養及寄主適應有關。同時, Arsenophonus還會誘髮草莓和甜菜上的病害。目前,A.nasoniae和R.pediculicola已經完成瞭基因組的測序工作。文章主要對Arsenophonus的研究進行瞭綜述,併對其未來的研究方嚮作瞭展望。
Arsenophonus대속우변형균문(Proteobacteria)γ아류장간균과(Enterobacteriaceae),시곤충、지주등체내상견공생세균,환견우첨채、초매등식물。해균재숙주곤충체내기능수직전파야능수평전파,재숙주곤충체내다정미산성분포,소수분포우특화적균포체。일반통과16S rDNA,23S rDNA적특이서렬혹기우fbaA, ftzK화yaeT 적다기인좌계통진행분자감정。흘금위지,지유5충Arsenophonus속적세균피감정,분별시Arsenophonus nasoniae,Arsenophonus arthropodicus,Arsenophonus triatominarum,Riesia pediculicola화Phlomobacter frageriae。 Arsenophonus대숙주곤충유살웅작용,환가능여숙주곤충적면역、영양급기주괄응유관。동시, Arsenophonus환회유발초매화첨채상적병해。목전,A.nasoniae화R.pediculicola이경완성료기인조적측서공작。문장주요대Arsenophonus적연구진행료종술,병대기미래적연구방향작료전망。
The genus Arsenophonus (γ-proteobacteria, Enterobacteriaceae ) is an intracellular symbiotic bacteria, which are commonly found in insects and spiders as well as in plants (strawberry, sugarbeet, etc).Arsenophonus could be vertical or horizontal transmitted to the hosts .The distribution of most Arsenophonus is usually diffused in insect, and seldom could be found in bacteriocytes .The specific sequence of 16S rDNA and 23S rDNA or multilocus system based on three housekeeping genes fbaA, ftzK and yaeT, which were used for identifying Arsenophonus.To date, only five species of Arsenophonus were identified, named Arsenophonus nasoniae, Arsenophonus arthropodicus, Arsenophonus triatominarum, Riesia pediculicola and Phlomobacter frageriae respectively.Previous studies revealed Arsenophonus is male killing and maybe associated with the immunity , nutrition requirement and host adaptation of its insect hosts.Strawberry and sugar beet infected by Arsenophonus would have diseases.Until now, genome sequencing of A.nasoniae and R.pediculicola were conducted and the drafts had been reported .This paper reviewed recent research progress of Arsenophonus.Furthermore, future research directions were also discussed .