中华普通外科学文献（电子版）
中華普通外科學文獻（電子版）
중화보통외과학문헌（전자판）
CHINESE JOURNAL OF GENERAL SURGERY(ELECTRONIC VERSION)
2014年
2期
102-105
,共4页
周健%郭志勇%鞠卫强%焦兴元%王小平%韩明%胡安斌%巫林伟%袁小鹏%马毅%朱晓峰%何晓顺
週健%郭誌勇%鞠衛彊%焦興元%王小平%韓明%鬍安斌%巫林偉%袁小鵬%馬毅%硃曉峰%何曉順
주건%곽지용%국위강%초흥원%왕소평%한명%호안빈%무림위%원소붕%마의%주효봉%하효순
部分肝切除%应用解剖%小鼠%模型%标准
部分肝切除%應用解剖%小鼠%模型%標準
부분간절제%응용해부%소서%모형%표준
Partial hepatectomy%Application anatomy%Mice%Model%Standard
目的观测C57BL/6J小鼠肝脏的解剖及建立70%、90%标准肝部分切除模型。方法60只成年SPF级C57BL/6J小鼠随机分3组。A组小鼠称量体质量、全肝质量及各肝叶质量，计算全肝质量/体质量比、70%和90%肝比例。采用直视下分步单纯丝线结扎肝脏解剖功能单位后再切除肝叶的方法，B组行70%肝切除；C组行90%肝切除。观察术后动物生存情况。结果小鼠全肝质量为（1.325±0.029）g，体质量为（26.5±1.14）g，左外侧叶质量为（0.433±0.041）g，中叶为（0.411±0.035）g，右前叶为（0.140±0.011）g，右后叶为（0.175±0.015）g，尾状叶为（0.099±0.006）g，全肝质量/体质量比为（4.92±0.13）%。肝左外侧叶与中叶之和约占全肝比重的70%；仅剩余尾状叶则切除肝比重约为90%。B组和C组手术成功率为100%，B组术后1周动物存活率为100%，C组术后1 d存活率为30%（6/20），2 d存活率为0。结论本研究首次明确小鼠各肝叶比例。采用直视下分步单纯丝线结扎肝脏解剖功能单位后再切除肝叶的方法，极易建立稳定、可靠的小鼠70%和90%标准肝部分切除模型。
目的觀測C57BL/6J小鼠肝髒的解剖及建立70%、90%標準肝部分切除模型。方法60隻成年SPF級C57BL/6J小鼠隨機分3組。A組小鼠稱量體質量、全肝質量及各肝葉質量，計算全肝質量/體質量比、70%和90%肝比例。採用直視下分步單純絲線結扎肝髒解剖功能單位後再切除肝葉的方法，B組行70%肝切除；C組行90%肝切除。觀察術後動物生存情況。結果小鼠全肝質量為（1.325±0.029）g，體質量為（26.5±1.14）g，左外側葉質量為（0.433±0.041）g，中葉為（0.411±0.035）g，右前葉為（0.140±0.011）g，右後葉為（0.175±0.015）g，尾狀葉為（0.099±0.006）g，全肝質量/體質量比為（4.92±0.13）%。肝左外側葉與中葉之和約佔全肝比重的70%；僅剩餘尾狀葉則切除肝比重約為90%。B組和C組手術成功率為100%，B組術後1週動物存活率為100%，C組術後1 d存活率為30%（6/20），2 d存活率為0。結論本研究首次明確小鼠各肝葉比例。採用直視下分步單純絲線結扎肝髒解剖功能單位後再切除肝葉的方法，極易建立穩定、可靠的小鼠70%和90%標準肝部分切除模型。
목적관측C57BL/6J소서간장적해부급건립70%、90%표준간부분절제모형。방법60지성년SPF급C57BL/6J소서수궤분3조。A조소서칭량체질량、전간질량급각간협질량，계산전간질량/체질량비、70%화90%간비례。채용직시하분보단순사선결찰간장해부공능단위후재절제간협적방법，B조행70%간절제；C조행90%간절제。관찰술후동물생존정황。결과소서전간질량위（1.325±0.029）g，체질량위（26.5±1.14）g，좌외측협질량위（0.433±0.041）g，중협위（0.411±0.035）g，우전협위（0.140±0.011）g，우후협위（0.175±0.015）g，미상협위（0.099±0.006）g，전간질량/체질량비위（4.92±0.13）%。간좌외측협여중협지화약점전간비중적70%；부잉여미상협칙절제간비중약위90%。B조화C조수술성공솔위100%，B조술후1주동물존활솔위100%，C조술후1 d존활솔위30%（6/20），2 d존활솔위0。결론본연구수차명학소서각간협비례。채용직시하분보단순사선결찰간장해부공능단위후재절제간협적방법，겁역건립은정、가고적소서70%화90%표준간부분절제모형。
Objective To observe anatomy of liver and establish 70%and 90%standard partial hepatectomy model in C57BL/6J mice. Methods Sixty adult mice with a C57BL/6J SPF stage were randomly equably divided into three groups, group A, group B and group C. Weight of body, total liver, and every single liver lobe were weighed in group A, ratio of total liver weight/body weight, and proportion of 70%and 90%liver was calculated, respectively. 70%partial hepatectomy in group B and 90%partial hepatectomy in group C were performed, using step by step visually ligaturing anatomic functional unit of liver by only threads. Results The weight of total liver, body, left lateral lobe, median lobe, right anterior lobe, right posterior lobe and caudate lobe in mice were (1.325±0.029) g, (26.500±1.140) g, (0.433±0.041) g, (0.411±0.035) g, (0.140±0.011) g, (0.175±0.015) g, (0.099±0.006) g, respectively, the ratio of total liver weight/body weight was (4.92±0.13)%, the weight of left lateral lobe and median lobe accounted for 70%of the whole liver, and the weight of other parts except caudate lobe preserved was 90%. The successful operation rate in both group B and C was 100%. The survival rate was 100%one week after operation in group B, 30%(6/20) one day after operation in group C, respectively, and no survival in group C two days after operation. Conclusions This study ascertains the proportion of every liver lobe in mice for the first time. Using step by step visually ligaturing anatomic functional unit of liver by only threads, it is easy to establish stable and credible 70%and 90%partial hepatectomy model in mice.
