环境保护科学
環境保護科學
배경보호과학
ENVIRONMENTAL PROTCTION SCIENCE
2014年
5期
61-64
,共4页
刘洞阳%李继%吕小梅%夏雪
劉洞暘%李繼%呂小梅%夏雪
류동양%리계%려소매%하설
胞外聚合物%聚磷污泥%电子受体%三维荧光光谱
胞外聚閤物%聚燐汙泥%電子受體%三維熒光光譜
포외취합물%취린오니%전자수체%삼유형광광보
Extracellular Polymeric Substances%Phosphorus Removal Sludge%Electron Acceptor%Three-dimensional Emission and Excitation Matrixs
采用3种电子受体（硝氮、亚硝氮、氧），在SBR反应器中分别驯化了具有稳定除磷能力的聚磷污泥。对比研究了不同聚磷污泥胞外聚合物（ EPS ）的组分与含量，结合三维荧光光谱对EPS中有机物质进行分析。研究表明，以硝氮、亚硝氮为电子受体的聚磷污泥EPS平均浓度较为接近，分别为115.6 mg/gVSS、133.5 mg/gVSS ，由厌氧段至缺氧段， EPS总浓度有所下降。以氧为电子受体聚磷污泥的EPS平均浓度相对较高，为194.5 mg/gVSS ，由厌氧段至好氧段， EPS总浓度略有升高。不同聚磷污泥的EPS组分均以胞外蛋白为主，占EPS总量的53%~65%，多糖与核酸占EPS总量28%~34%、5%~7%。三维荧光光谱显示3种聚磷污泥EPS均具有芳香结构蛋白质、可溶性代谢物的荧光峰，另外，以硝氮为电子受体的聚磷污泥还具有明显的腐殖酸类物质的荧光峰。研究结果表明电子受体对聚磷污泥EPS的组分、浓度、有机质类别有一定的影响。
採用3種電子受體（硝氮、亞硝氮、氧），在SBR反應器中分彆馴化瞭具有穩定除燐能力的聚燐汙泥。對比研究瞭不同聚燐汙泥胞外聚閤物（ EPS ）的組分與含量，結閤三維熒光光譜對EPS中有機物質進行分析。研究錶明，以硝氮、亞硝氮為電子受體的聚燐汙泥EPS平均濃度較為接近，分彆為115.6 mg/gVSS、133.5 mg/gVSS ，由厭氧段至缺氧段， EPS總濃度有所下降。以氧為電子受體聚燐汙泥的EPS平均濃度相對較高，為194.5 mg/gVSS ，由厭氧段至好氧段， EPS總濃度略有升高。不同聚燐汙泥的EPS組分均以胞外蛋白為主，佔EPS總量的53%~65%，多糖與覈痠佔EPS總量28%~34%、5%~7%。三維熒光光譜顯示3種聚燐汙泥EPS均具有芳香結構蛋白質、可溶性代謝物的熒光峰，另外，以硝氮為電子受體的聚燐汙泥還具有明顯的腐殖痠類物質的熒光峰。研究結果錶明電子受體對聚燐汙泥EPS的組分、濃度、有機質類彆有一定的影響。
채용3충전자수체（초담、아초담、양），재SBR반응기중분별순화료구유은정제린능력적취린오니。대비연구료불동취린오니포외취합물（ EPS ）적조분여함량，결합삼유형광광보대EPS중유궤물질진행분석。연구표명，이초담、아초담위전자수체적취린오니EPS평균농도교위접근，분별위115.6 mg/gVSS、133.5 mg/gVSS ，유염양단지결양단， EPS총농도유소하강。이양위전자수체취린오니적EPS평균농도상대교고，위194.5 mg/gVSS ，유염양단지호양단， EPS총농도략유승고。불동취린오니적EPS조분균이포외단백위주，점EPS총량적53%~65%，다당여핵산점EPS총량28%~34%、5%~7%。삼유형광광보현시3충취린오니EPS균구유방향결구단백질、가용성대사물적형광봉，령외，이초담위전자수체적취린오니환구유명현적부식산류물질적형광봉。연구결과표명전자수체대취린오니EPS적조분、농도、유궤질유별유일정적영향。
Three kinds of electron acceptors including nitrate, nitrite and oxygen were used to acclimate the phosphorus removal sludge with steady phosphorus removal capacity in sequencing batch reactors (SBR) respectively. Components and concentrations of extracellular polymer substances (EPS) of different phosphorus removal sludge were compared and organic matters in the EPS were analyzed with three-dimensional emission and excitation matrixs (EEM). The results showed that the average concentration of EPS acclimated with nitrate and nitrite as the electron acceptors was 115.6 mg/gVSS and 133.5 mg/gVSS, which appeared almost on the same level. However, the total concentration of EPS decreased when the environment was shifted from anaerobic condition to anoxic condition. The average concentration of EPS acclimated with oxygen as the electron acceptor was 194.5 mg/gVSS, which appeared comparatively higher, and the total concentration of EPS increased slightly when the environment was shifted from anaerobic condition to aerobic condition. With regard to the components of EPS of different phosphorus removal sludge, exoprotain occupied the majority, accounting for 53%~65% of the total. Polysaccharide and nucleic acid accounted for 28%~34% and 5%~7% respectively. It was indicated in the three-dimensional EEM that EPS of all the three phosphorus removal sludge with different electron acceptors had the fluorescence peaks for aromatic protein and soluble metabolites. Moreover, EPS of the phosphorus removal sludge with nitrate as the electron acceptor had the obvious fluorescence peak for humic acids. Therefore, it could be concluded that electron acceptors had certain effects on components, concentrations and organic matters of the EPS of phosphorus removal sludge.
