CAJ | 학술논문

目的探讨法舒地尔对大鼠低氧性肺动脉高压及肺组织中bFGF、VEGF表达的影响。方法雄性Wistar大鼠24只随机分为常氧对照组、低氧模型组、低氧法舒地尔干预组，检测各组平均肺动脉压力( mPAP)、右心室肥厚指数( RVHI)；光镜下观察大鼠肺血管形态结构；RT-PCR检测bFGFmRNA、VEGFmRNA的变化。结果低氧模型组mPAP、RVHI值明显高于常氧对照组(P<0.01)；法舒地尔干预组 mPAP、RVHI显著低于低氧模型组(P<0.05)。光镜示，法舒地尔干预后大鼠的肺血管形态学改变较低氧组明显缓解。RT-PCR示，低氧模型组bFGF、VEGF值与其他两组比较明显升高(P<0.05)。结论法舒地尔对低氧所致的肺动脉高压、右心室肥厚及肺血管重塑疗效显著；bFGF、VEGF参与肺血管重塑的过程，其表达水平与肺血管重塑严重程度相关。
목적탐토법서지이대대서저양성폐동맥고압급폐조직중bFGF、VEGF표체적영향。방법웅성Wistar대서24지수궤분위상양대조조、저양모형조、저양법서지이간예조，검측각조평균폐동맥압력( mPAP)、우심실비후지수( RVHI)；광경하관찰대서폐혈관형태결구；RT-PCR검측bFGFmRNA、VEGFmRNA적변화。결과저양모형조mPAP、RVHI치명현고우상양대조조(P<0.01)；법서지이간예조 mPAP、RVHI현저저우저양모형조(P<0.05)。광경시，법서지이간예후대서적폐혈관형태학개변교저양조명현완해。RT-PCR시，저양모형조bFGF、VEGF치여기타량조비교명현승고(P<0.05)。결론법서지이대저양소치적폐동맥고압、우심실비후급폐혈관중소료효현저；bFGF、VEGF삼여폐혈관중소적과정，기표체수평여폐혈관중소엄중정도상관。
Objective To study the effect of fasudil on hypoxic pulmonary hypertension and the expression of bFGF and VEGF in lung tissue of hypoxic pulmonary hypertension in rats. Method Twenty-four male Wistar rats were di-vided randomly into 3 groups:normal control group,hypoxic model group and fasudil treatment group. We measured pulmonary arterial press and right ventricle hypertrophy index. The pulmonary artery morphological changes were ob-served under light microscope;basic fibroblast growth factor mRNA and Vascular endothelial growth factor mRNA were detectd by Reverse Transcriptase Polymerase Chain Reaction to the changes of. Result The level of mPAP and RVHI were significantlly higher in the hypoxic model group than those in normal control group and those of fasudil treated group(P<0. 05). There were obviously morphological changes of pulmonary vascular that were alleviated in the fasudil treatment group than those in lower oxygen model group on Light microscopy. The mRNA expression of bFGF and VEGF were significantly increased than those in the the fasudil treatment group,and the normal control group (P<0. 01). Conclusion Fasudil showed significantlly effective to prevent and reverse the development of pul-monary hypertension and right ventricular hypertrophy and pulmonary vascular from remodeling. bFGF and VEGF participate in the process of pulmonary vascular remodeling. The expression level of bFGF and VEGF relate directly to pulmonary vascular remodeling in severity.