中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2013年
5期
54-58
,共5页
石蕊寒%丁叶%佘锐萍%朱金凤%常玲玲%尤华
石蕊寒%丁葉%佘銳萍%硃金鳳%常玲玲%尤華
석예한%정협%사예평%주금봉%상령령%우화
三聚氰胺%SPF昆明小鼠%睾丸%组织病理学%免疫组织化学
三聚氰胺%SPF昆明小鼠%睪汍%組織病理學%免疫組織化學
삼취청알%SPF곤명소서%고환%조직병이학%면역조직화학
Melamine%SPF Kunming mouse%Testis%Histopathology%Immunohistochemical
本研究采用组织病理学和免疫组织化学技术观察了三聚氰胺(Melamine,MA)对雄性小鼠睾丸的毒性作用,以深入揭示三聚氰胺的毒性作用及其机理。将25只28日龄SPF雄性昆明小鼠随机均分为5组,1组为对照组,其他4组为试验组。4个试验组分别按下述不同剂量灌服三聚氰胺:0.6 mg/kg(bw)/d(每日每公斤体重0.6 mg)、3.0 mg/kg(bw)/d、15.0 mg/kg(bw)/d和17.5 mg/kg(bw)/d,用玉米油做溶剂;空白对照组每日灌服等量玉米油。连续灌胃30天后剖检小鼠并取其睾丸组织进行固定、切片和染色,观察睾丸的组织病理变化。同时,采用免疫组织化学方法检测睾丸组织中增殖细胞核抗原(PCNA)的表达量。观察结果表明,与对照组相比,试验组不同剂量浓度的三聚氰胺对小鼠睾丸组织造成不同程度的病理损伤;三聚氰胺也会在一定程度上影响睾丸组织中PCNA的表达。研究结果表明三聚氰胺对于小鼠生殖系统有明显毒性作用。
本研究採用組織病理學和免疫組織化學技術觀察瞭三聚氰胺(Melamine,MA)對雄性小鼠睪汍的毒性作用,以深入揭示三聚氰胺的毒性作用及其機理。將25隻28日齡SPF雄性昆明小鼠隨機均分為5組,1組為對照組,其他4組為試驗組。4箇試驗組分彆按下述不同劑量灌服三聚氰胺:0.6 mg/kg(bw)/d(每日每公斤體重0.6 mg)、3.0 mg/kg(bw)/d、15.0 mg/kg(bw)/d和17.5 mg/kg(bw)/d,用玉米油做溶劑;空白對照組每日灌服等量玉米油。連續灌胃30天後剖檢小鼠併取其睪汍組織進行固定、切片和染色,觀察睪汍的組織病理變化。同時,採用免疫組織化學方法檢測睪汍組織中增殖細胞覈抗原(PCNA)的錶達量。觀察結果錶明,與對照組相比,試驗組不同劑量濃度的三聚氰胺對小鼠睪汍組織造成不同程度的病理損傷;三聚氰胺也會在一定程度上影響睪汍組織中PCNA的錶達。研究結果錶明三聚氰胺對于小鼠生殖繫統有明顯毒性作用。
본연구채용조직병이학화면역조직화학기술관찰료삼취청알(Melamine,MA)대웅성소서고환적독성작용,이심입게시삼취청알적독성작용급기궤리。장25지28일령SPF웅성곤명소서수궤균분위5조,1조위대조조,기타4조위시험조。4개시험조분별안하술불동제량관복삼취청알:0.6 mg/kg(bw)/d(매일매공근체중0.6 mg)、3.0 mg/kg(bw)/d、15.0 mg/kg(bw)/d화17.5 mg/kg(bw)/d,용옥미유주용제;공백대조조매일관복등량옥미유。련속관위30천후부검소서병취기고환조직진행고정、절편화염색,관찰고환적조직병리변화。동시,채용면역조직화학방법검측고환조직중증식세포핵항원(PCNA)적표체량。관찰결과표명,여대조조상비,시험조불동제량농도적삼취청알대소서고환조직조성불동정도적병리손상;삼취청알야회재일정정도상영향고환조직중PCNA적표체。연구결과표명삼취청알대우소서생식계통유명현독성작용。
Histopathological and immunohistochemical techniques were used to investigate subchronic toxic injury caused by melamine on testis of SPF male Kunming mice in order to reveal the toxic effects of melamine and its mechanism. Twenty-five 28-day-old SPF male Kunming mice were randomly and evenly divided into 5 groups,of which 4 were for trial use and the another one for control. Dissolved in corn oil,different doses of melamine,0.6 mg/kg(bw)/d、3.0 mg/kg(bw)/d、15.0 mg/kg(bw)/d and 17.5 mg/kg(bw)/d,were given respectively to the 4 trial groups in gavage,while the control group received equal quantity of corn oil for 30 days. Paraffin section technique was employed to observe the testicular histopathological changes and immunohistochemical method was applied to detect the expression level of PCNA(proliferating cell nuclear antigen). Results demonstrated that,compared with the control group,the 4 test groups were found to suffer testicular pathological injuries in different degrees,varying with melamine doses and melamine also affected the expression of PCNA to some certain extent. Evident toxic effects of MA on mice reproductive system were revealed in this study.