CAJ | 학술논문

　　用5种不同的培养基对胶州湾近岸污染环境可培养半知菌进行选择性分离,共得到235株半知菌,其中YM培养基、MPDA培养基对半知菌的分离效果最好,分别占分离总量的26.4%和24.7%。试验选择的3个站位：石老人潮间带、海泊河入海口、李村河入海口PAHs含量存在显著性差异(P<0.05),其中石老人最低,平均为113.8 ng/g,海泊河 PAHs 为324.9 ng/g,介于中等水平,李村河含量最高为882.1 ng/g。可培养半知菌群体随PAHs含量的增加表现出先升高后降低的趋势。对各站位的半知菌进行统计发现,污染不同的站位,可培养半知菌优势种群不同,说明PAHs的污染可对半知菌的菌落结构组成产生影响。
　　용5충불동적배양기대효주만근안오염배경가배양반지균진행선택성분리,공득도235주반지균,기중YM배양기、MPDA배양기대반지균적분리효과최호,분별점분리총량적26.4%화24.7%。시험선택적3개참위：석노인조간대、해박하입해구、리촌하입해구PAHs함량존재현저성차이(P<0.05),기중석노인최저,평균위113.8 ng/g,해박하 PAHs 위324.9 ng/g,개우중등수평,리촌하함량최고위882.1 ng/g。가배양반지균군체수PAHs함량적증가표현출선승고후강저적추세。대각참위적반지균진행통계발현,오염불동적참위,가배양반지균우세충군불동,설명PAHs적오염가대반지균적균락결구조성산생영향。
Five different kinds of culture medium were used to selectively isolate culturable Deuteromycetes in contaminated coast of Jiaozhou bay and 235 strains of Deuteromycetes were obtained. Among the medium, YM and MPDA isolated Deuteromycetes the best. The isolate rates of the total isolated-strains of YM and MPDA were 26.4% and 24.7%, respectively. PAHs contents among the three sampling sites manifested significant difference (P<0.05) with the lowest in Shilaoren tidal zone (average 113.8ng/g), moderate in Haibo River estuary (average 324.9 ng/g) and the highest in Licun River estuary (average 882.1ng/g). On the basis of the changes of PAHs con-tents in different sites, with the increase of PAHs amount, the amount of culturable Deuteromycetes increased in certain degree and then decreased. Further research showed that the dominant species differed in the three sites with different PAHs contents which indicated PAHs contamination might exert effect on the composition of Deutero-mycetes colony.