CAJ | 학술논문

　　利用硅胶色谱、Sephadex LH-20凝胶色谱、HPLC等技术,从坛紫菜(Porphyra haitanensis)乙醇提取物首次分离纯化得到了7个化合物,通过质谱(MS)及核磁共振波谱(1H NMR、13C NMR)等现代波谱技术鉴定为：1,8-二羟基-9,10-蒽醌(1)、邻苯二甲酸二正丁酯(2)、邻苯二甲酸二异丁酯(3)、β-谷甾醇(4)、鲨肝醇(5)、(E)-N-2(1,3-二羟基-4-十八烯基)-十六酰胺(6)、胆甾醇(7)。其中,化合物1对金黄色葡萄球菌(Staphyloccocus aureus)的最低抑菌浓度(MIC)为80μg/mL。
　　이용규효색보、Sephadex LH-20응효색보、HPLC등기술,종단자채(Porphyra haitanensis)을순제취물수차분리순화득도료7개화합물,통과질보(MS)급핵자공진파보(1H NMR、13C NMR)등현대파보기술감정위：1,8-이간기-9,10-은곤(1)、린분이갑산이정정지(2)、린분이갑산이이정지(3)、β-곡치순(4)、사간순(5)、(E)-N-2(1,3-이간기-4-십팔희기)-십륙선알(6)、담치순(7)。기중,화합물1대금황색포도구균(Staphyloccocus aureus)적최저억균농도(MIC)위80μg/mL。
Compounds1~7 were isolated from Porphyra haitanensis by normal phase silica gel, Sephadex LH-20 chromatography, and reverse phase HPLC techniques. Their structures were identified as 1,8-dihydroxy- an-thraquinone(1), dibutyl-O-phthalate(2), dibutyl phthalate(3), sitosterol(4), batyl alcohol(5), (E)-N-2-(1,3-dihydroxy octadecan-4-en)-hexadecamide(6) and cholesterol (7) using MS and NMR. The MIC of compound 1 towards Staphyloccocus aureus is 80μg.ml-1. Compounds 1~7 were found in Porphyra for the first time.