CAJ | 학술논문

目的：探讨基因芯片技术在阴道分泌物常规检测中的应用价值。方法选取2012年1-4月首都医科大学附属北京天坛医院妇产科门诊400例患者阴道分泌物，每份标本同时用 BD Affirm VPⅢ芯片检测、显微镜镜检、细菌性阴道病（BV）甲酚蓝试剂检测和 BV 蓝试剂检测，并接种至念珠菌显色培养基和滴虫培养基培养。采用 SPSS 16.0统计软件，行 Kappa 检验。结果以 BV 蓝试剂检测结果为金标准，BV 甲酚蓝试剂检测和 BD Affirm VPⅢ芯片检测 BV的 Kappa 值分别为0.813和0.553。以念珠菌培养结果为金标准，显微镜镜检和 BD Affirm VPⅢ芯片检测念珠菌的 Kap-pa 值分别为0.605和0.941。以滴虫培养结果为金标准，显微镜镜检和 BD Affirm VPⅢ芯片检测滴虫的 Kappa 值分别为0.973和0.923。结论 BD Affirm VPⅢ芯片检测念珠菌和滴虫一致性较好，BD Affirm VPⅢ芯片与 BV 蓝或 BV 甲酚蓝试剂联合用于临床进行阴道分泌物检查，可以取代显微镜镜检。
목적：탐토기인심편기술재음도분비물상규검측중적응용개치。방법선취2012년1-4월수도의과대학부속북경천단의원부산과문진400례환자음도분비물，매빈표본동시용 BD Affirm VPⅢ심편검측、현미경경검、세균성음도병（BV）갑분람시제검측화 BV 람시제검측，병접충지념주균현색배양기화적충배양기배양。채용 SPSS 16.0통계연건，행 Kappa 검험。결과이 BV 람시제검측결과위금표준，BV 갑분람시제검측화 BD Affirm VPⅢ심편검측 BV적 Kappa 치분별위0.813화0.553。이념주균배양결과위금표준，현미경경검화 BD Affirm VPⅢ심편검측념주균적 Kap-pa 치분별위0.605화0.941。이적충배양결과위금표준，현미경경검화 BD Affirm VPⅢ심편검측적충적 Kappa 치분별위0.973화0.923。결론 BD Affirm VPⅢ심편검측념주균화적충일치성교호，BD Affirm VPⅢ심편여 BV 람혹 BV 갑분람시제연합용우림상진행음도분비물검사，가이취대현미경경검。
Objective To study the application of gene chip technique in leucorrhea routine examination. Methods Vaginal secretions of 400 gynecological outpatients referred to our hospital from January 2012 to April 2012 were tested with BD Affirm VPⅢ chips,microscopes,BV cresol blue agent and BV blue agent for testing bacterial vaginopathy respectively,which were cultured in candida color medium and trichomonad culture mediums. Kappa tests were performed with the statistical program SPSS 16. 0. Results The results of BV blue reagent were regarded as golden standard for testing bacterial vaginopathy,the Kap-pa values of BV cresol blue reagent and BD Affirm VPⅢ chips were 0. 813 and 0. 553 respectively. The results of candida chromo-genic plates were regarded as golden standard for testing candida,the Kappa values of microscope and BD Affirm VPⅢ chips were 0. 605 and 0. 941 respectively. The results of trichomonad culture mediums were regarded as golden standard for testing trich-omonad,the Kappa values of microscope and BD Affirm VPⅢ chips were 0. 973 and 0. 923 respectively. Conclusion The BD Affirm VPⅢ gene chips accompanied with BV blue reagent or BV cresol blue reagent can take the place of microscope examination to test vaginal discharge clinically.