CAJ | 학술논문

　　目的：研究黑乳海参（Holothuria nobilis Selenka）中硫酸化多糖类型、理化性质及抗凝活性。方法：利用化学和物理相结合的分离方法进行纯化，采用色谱、化学、光谱等方法对其纯度、化学组成、分子量、特性黏度、旋光度、凝血活性等进行分析。结果：分离获得F-I、F-II两种多糖组分。F-I由葡萄糖醛酸、乙酰氨基半乳糖、岩藻糖和硫酸基（摩尔比1.00：0.96：1.18：2.99）组成；F-II仅由岩藻糖和硫酸基（摩尔比1.00：0.51）；它们的理化性质显著不同，前者的抗凝效价约为后者的6倍。结论：黑乳海参中至少存在两种不同的硫酸化多糖，岩藻糖化糖胺聚糖和α-L-硫酸化岩藻聚糖，均具有强的抗凝血活性。
　　목적：연구흑유해삼（Holothuria nobilis Selenka）중류산화다당류형、이화성질급항응활성。방법：이용화학화물리상결합적분리방법진행순화，채용색보、화학、광보등방법대기순도、화학조성、분자량、특성점도、선광도、응혈활성등진행분석。결과：분리획득F-I、F-II량충다당조분。F-I유포도당철산、을선안기반유당、암조당화류산기（마이비1.00：0.96：1.18：2.99）조성；F-II부유암조당화류산기（마이비1.00：0.51）；타문적이화성질현저불동，전자적항응효개약위후자적6배。결론：흑유해삼중지소존재량충불동적류산화다당，암조당화당알취당화α-L-류산화암조취당，균구유강적항응혈활성。
Objective: To study the polysaccharide types, physicochemical properties and anticoagulant activities of sulfated polysaccharides from the sea cucumber Holothuria nobilis Selenka. Methods: Sulfated polysaccharides were purified by methods of chemistry and physics. Physicochemical properties and biological activities of the saccharides, such as purity, chemical composition, molecular weight, intrinsic viscosity, optical rotation and anticoagulant activities were determined by methods of chromatography, chemistry, spectrum technologies, etc. Results: The polysaccharides were separated into two fractions, F-I and F-II, respectively. The components of F-I were identified as glucuronic acid, aminogalactose, fucan and sulfate (molar ratio 1.00:0.96:1.18:2.99); F-II contained only fucan and sulfate (molar ratio 1.00:0.51). The two polysaccharides were significantly different in the physicochemical properties, and F-I had 6 times of anticoagulant potency compared to the F-II. Conclusion: The sea cucumber H. nobilis contains at least two types of sulfated polysaccharides, a fucosylated glycosaminoglycan sulfate and a sulfated α -L -fucan which have anticoagulant activities.