CAJ | 학술논문

　　目的建立高效液相色谱（HPLC）方法同时测定中药金银花中咖啡酸及木犀草苷的含量。方法采用Agilent C18色谱柱（250mm×4.6mm，5.0μm），流动相采用甲醇：0.1%的磷酸水溶液，按照梯度洗脱方式，流速为1.0ml/min，进样量为15μl，检测波长为338nm，柱温为25℃。结果咖啡酸与木犀草苷分别在8.1-82.0μg/ml、2.1-42.0μg/ml范围内线性关系良好，线性回归方程分别为Y=37.71X-265.77（R=0.9997），Y=15.56X+3.98（R=0.9995）。结论采用HPLC方法测定金银花中的有效成分咖啡酸、木犀草苷，方法简单、分离效果好，重复性好，是一种较好的检测金银花药材质量的方式。
　　목적건립고효액상색보（HPLC）방법동시측정중약금은화중가배산급목서초감적함량。방법채용Agilent C18색보주（250mm×4.6mm，5.0μm），류동상채용갑순：0.1%적린산수용액，안조제도세탈방식，류속위1.0ml/min，진양량위15μl，검측파장위338nm，주온위25℃。결과가배산여목서초감분별재8.1-82.0μg/ml、2.1-42.0μg/ml범위내선성관계량호，선성회귀방정분별위Y=37.71X-265.77（R=0.9997），Y=15.56X+3.98（R=0.9995）。결론채용HPLC방법측정금은화중적유효성분가배산、목서초감，방법간단、분리효과호，중복성호，시일충교호적검측금은화약재질량적방식。
Objective To establish high performance liquid chromatography(HPLC) in the determination of the content of Flos Lonicerae in coffee acid and galuteolin. Methods Taking Agilent C18 column(250mm×4.6mm, 5μm), mobile phase of methanol: water solution of phosphoric acid by 0.1%, according to the gradient elution mode, the flow rate was 1.0ml/min, the injection volume was 15μl, the detection wavelength was 338nm, the column temperature was 25℃ . Results Coffee acid and galuteolin respectively in 8.1-82.0μg/ml, 2.1-42.0μg/ml was with good linear relationship, the linear regression equation was Y=37.71X-265.77(R=0.9997), Y=15.56X+3.98(R=0.9995). Conclusion The method of HPLC method in the determination of coffee acid in Flos Lonicerae Japonicae, galuteolin is simple, and with good separation effect, good repeatability, and it is a better detection of Flos Lonicerae Japonicae quality.