中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2013年
13期
770-774
,共5页
王贺%孙风波%张凤娟%亓玉琴
王賀%孫風波%張鳳娟%亓玉琴
왕하%손풍파%장봉연%기옥금
Tiam-1%HPA-1%肝癌%侵袭转移%实时荧光定量PCR
Tiam-1%HPA-1%肝癌%侵襲轉移%實時熒光定量PCR
Tiam-1%HPA-1%간암%침습전이%실시형광정량PCR
Tiam-1%HPA-1%hepatocellular carcinoma%invasion and metastasis%real-time fluorescent quantitative reverse transcriptase polymerase chain reaction
目的：探讨Tiam-1和HPA-1基因在肝癌中的定量表达及其与肝癌侵袭转移的关系。方法：选取2009年5月至2012年1月行手术治疗的肝癌患者65例。采用实时荧光定量逆转录聚合酶链反应（RT-PCR）方法检测 Tiam-1 mRNA 及 HPA-1 mRNA在原发性肝细胞肝癌、配对癌旁组织（距癌2 cm）及正常肝脏组织中（距癌>5 cm）的表达水平，并分析两者与肝癌临床病理参数的关系。结果：肝癌组织中Tiam-1 mRNA的表达水平（43.83±11.62）显著高于癌旁组织（14.82±8.16）和正常肝脏组织（6.02±5.36）（均 P<0.05），癌旁组织中 Tiam-1 mRNA 的表达水平高于正常肝脏组织（P<0.05）。肝癌组织中 HPA-1 mRNA 的表达水平（35.28±11.81）显著高于癌旁组织（12.94±6.25）和正常肝脏组织（4.17±3.49）（均P<0.05），癌旁组织中HPA-1 mRNA的表达水平高于正常肝脏组织（P<0.05）。Tiam-1及HPA-1的表达增强与肿瘤的分化程度、浸润深度、淋巴结转移、血行转移及TNM分期显著相关（均 P<0.05）。Spearman 等级相关分析证明了 Tiam-1和 HPA-1的表达呈明显正相关（OR=0.523，P<0.05）。结论：Tiam-1 mRNA和HPA-1 mRNA在肝癌组织中呈高表达，且两者的表达增强可促进肝癌的侵袭转移。联合检测Tiam-1及HPA-1对于指导肝癌临床治疗及判断预后有重要价值。
目的：探討Tiam-1和HPA-1基因在肝癌中的定量錶達及其與肝癌侵襲轉移的關繫。方法：選取2009年5月至2012年1月行手術治療的肝癌患者65例。採用實時熒光定量逆轉錄聚閤酶鏈反應（RT-PCR）方法檢測 Tiam-1 mRNA 及 HPA-1 mRNA在原髮性肝細胞肝癌、配對癌徬組織（距癌2 cm）及正常肝髒組織中（距癌>5 cm）的錶達水平，併分析兩者與肝癌臨床病理參數的關繫。結果：肝癌組織中Tiam-1 mRNA的錶達水平（43.83±11.62）顯著高于癌徬組織（14.82±8.16）和正常肝髒組織（6.02±5.36）（均 P<0.05），癌徬組織中 Tiam-1 mRNA 的錶達水平高于正常肝髒組織（P<0.05）。肝癌組織中 HPA-1 mRNA 的錶達水平（35.28±11.81）顯著高于癌徬組織（12.94±6.25）和正常肝髒組織（4.17±3.49）（均P<0.05），癌徬組織中HPA-1 mRNA的錶達水平高于正常肝髒組織（P<0.05）。Tiam-1及HPA-1的錶達增彊與腫瘤的分化程度、浸潤深度、淋巴結轉移、血行轉移及TNM分期顯著相關（均 P<0.05）。Spearman 等級相關分析證明瞭 Tiam-1和 HPA-1的錶達呈明顯正相關（OR=0.523，P<0.05）。結論：Tiam-1 mRNA和HPA-1 mRNA在肝癌組織中呈高錶達，且兩者的錶達增彊可促進肝癌的侵襲轉移。聯閤檢測Tiam-1及HPA-1對于指導肝癌臨床治療及判斷預後有重要價值。
목적：탐토Tiam-1화HPA-1기인재간암중적정량표체급기여간암침습전이적관계。방법：선취2009년5월지2012년1월행수술치료적간암환자65례。채용실시형광정량역전록취합매련반응（RT-PCR）방법검측 Tiam-1 mRNA 급 HPA-1 mRNA재원발성간세포간암、배대암방조직（거암2 cm）급정상간장조직중（거암>5 cm）적표체수평，병분석량자여간암림상병리삼수적관계。결과：간암조직중Tiam-1 mRNA적표체수평（43.83±11.62）현저고우암방조직（14.82±8.16）화정상간장조직（6.02±5.36）（균 P<0.05），암방조직중 Tiam-1 mRNA 적표체수평고우정상간장조직（P<0.05）。간암조직중 HPA-1 mRNA 적표체수평（35.28±11.81）현저고우암방조직（12.94±6.25）화정상간장조직（4.17±3.49）（균P<0.05），암방조직중HPA-1 mRNA적표체수평고우정상간장조직（P<0.05）。Tiam-1급HPA-1적표체증강여종류적분화정도、침윤심도、림파결전이、혈행전이급TNM분기현저상관（균 P<0.05）。Spearman 등급상관분석증명료 Tiam-1화 HPA-1적표체정명현정상관（OR=0.523，P<0.05）。결론：Tiam-1 mRNA화HPA-1 mRNA재간암조직중정고표체，차량자적표체증강가촉진간암적침습전이。연합검측Tiam-1급HPA-1대우지도간암림상치료급판단예후유중요개치。
Objective: Invasion and metastasis are the main causes of carcinoma mortality; hence, the timely blocking of the invasion and metastasis of carcinoma has become a research hotspot. The present study aims to investigate the expression levels of Tiam-1 mRNA and HPA-1 mRNA and their correlation with the invasion and metastasis of hepatocellular carcinoma. Methods: From May 2009 to Jan 2012, 65 hepatocellular carcinoma patients admitted consecutively in our hospital for surgical treatment were included in this study. Real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to investigate the expression levels of Tiam-1 mRNA and HPA-1 mRNA in hepatocellular carcinoma, paired adjacent hepatocellular carcinoma (2 cm from the carcinoma), and surgical marginal normal hepato mucosa tissues (5 cm from the carcinoma). RT-PCR was also used to analyze their correlation with the clinicopathological characteristics of hepatocellular carcinoma. Results: The expression level of HPA-1 mRNA was significantly higher in hepatocellular carcinoma (43.83±11.62) than in paired adjacent hepatocellular carcinoma (14.82±8.16) and normal hepato mucosa tissues (6.02±5.36) (P<0.001). The expression level of HPA-1 mRNA was higher in paired adjacent hepatocellular carcinoma than in normal hepato mucosa tissues (P<0.05). The expression of Tiam-1 mRNA was higher in hepatocellular carcinoma (35.28±11.81) than in paired adjacent hepatocellular carcinoma (12.94±6.25) and normal hepato mucosa tissues (4.17±3.49) (P<0.05). The expression level of Tiam-1 mRNA was higher in paired adjacent hepatocellular carcinoma than in normal hepato mucosa tissues (P<0.05). The expression levels of Tiam-1 mRNA and HPA-1 mRNA were closely associated with the degree of differentiation, depth of infiltration, lymph node metastasis, vessel metastasis, and TNM (Tumor, Node, Metastasis)staging of gastric carcinoma (P<0.05). Spearman rank correlation analysis demonstrated a significant correlation between Tiam-1 and HPA-1 (OR=0.523, P<0.05). Conclusion: The expression levels of Tiam-1 mRNA and HPA-1 mRNA were high in hepatocellular carcinoma. Meanwhile, the increased ex-pression levels of Tiam-1 and HPA-1 can promote the invasion and metastasis of hepatocellular carcinoma. Moreover, the determination of Tiam-1 and HPA-1 may be valuable for the treatment and prognosis of hepatocellular carcinoma.
