饲料工业
飼料工業
사료공업
FEED INDUSTRY
2013年
13期
32-34
,共3页
红曲霉%酵母菌%混合发酵%最优条件
紅麯黴%酵母菌%混閤髮酵%最優條件
홍곡매%효모균%혼합발효%최우조건
Monascus%yeast%mixed fermentation%optimal conditions
通过菌种间相容性试验观察混菌相容情况，采用单因素筛选的方法，确定红曲霉、酵母菌混菌发酵的最优培养条件。结果表明：红曲霉和酵母菌可以兼容共生，但由于两菌种在相同环境下生长周期和生长能力不同而有一定拮抗作用，通过混合菌种优化条件的筛选，能降低拮抗，达到最佳效果。最优培养条件：在PDA培养基上,接种比例为（红曲霉?酵母菌）3?1,在32℃的环境温度下，在红曲霉接种培养4 d时接种酵母菌混合发酵至第7 d。
通過菌種間相容性試驗觀察混菌相容情況，採用單因素篩選的方法，確定紅麯黴、酵母菌混菌髮酵的最優培養條件。結果錶明：紅麯黴和酵母菌可以兼容共生，但由于兩菌種在相同環境下生長週期和生長能力不同而有一定拮抗作用，通過混閤菌種優化條件的篩選，能降低拮抗，達到最佳效果。最優培養條件：在PDA培養基上,接種比例為（紅麯黴?酵母菌）3?1,在32℃的環境溫度下，在紅麯黴接種培養4 d時接種酵母菌混閤髮酵至第7 d。
통과균충간상용성시험관찰혼균상용정황，채용단인소사선적방법，학정홍곡매、효모균혼균발효적최우배양조건。결과표명：홍곡매화효모균가이겸용공생，단유우량균충재상동배경하생장주기화생장능력불동이유일정길항작용，통과혼합균충우화조건적사선，능강저길항，체도최가효과。최우배양조건：재PDA배양기상,접충비례위（홍곡매?효모균）3?1,재32℃적배경온도하，재홍곡매접충배양4 d시접충효모균혼합발효지제7 d。
Through the strains compatibility tests to observe the mixed compatible,adopt single factor method of screening, determine the optimal cultivation condition of mixed bacteria fermen?tation with monascus and yeast. The results show that: monascus and yeast can be compatible with symbiosis, but because the two strains' grow ability and growth cycle are different in the same environment and have antagonist role, through the screening of the optimal conditions, an?tagonist can be reduced, achieving the best effect. The best culture conditions: On PDA medi?um, mixed bacteria vaccination scale is (monascus?yeast) 3?1, in 32 ℃ ambient temperature, monascus inoculation cultured 4 days and then yeast inoculation cultured to 7 days.
