CAJ | 학술논문

　　实验旨在研究蛋氨酸脑啡肽(MENK)对禽马立克细胞MSB-1生长抑制作用和凋亡诱导效应。用MENK对禽马立克细胞MSB-1细胞株进行干预，采用光镜观察细胞密度及形态，MTT法测定细胞增殖抑制率，流式细胞术检测细胞凋亡。镜下观察可见在10-12 mol/l MENK的作用下，MSB-1细胞株数量降低，细胞形态渐圆钝，甚至出现皱缩，与对照组相比MENK对MSB-1细胞具有明显的生长抑制作用，抑制率为19%~46%(P<0.01)，诱导细胞凋亡率为（62.04±3.32）%(P<0.01)。MENK对MSB-1细胞株具有增殖抑制效应及凋亡诱导效应，是具有发展潜力的抗肿瘤药物。
　　실험지재연구단안산뇌배태(MENK)대금마립극세포MSB-1생장억제작용화조망유도효응。용MENK대금마립극세포MSB-1세포주진행간예，채용광경관찰세포밀도급형태，MTT법측정세포증식억제솔，류식세포술검측세포조망。경하관찰가견재10-12 mol/l MENK적작용하，MSB-1세포주수량강저，세포형태점원둔，심지출현추축，여대조조상비MENK대MSB-1세포구유명현적생장억제작용，억제솔위19%~46%(P<0.01)，유도세포조망솔위（62.04±3.32）%(P<0.01)。MENK대MSB-1세포주구유증식억제효응급조망유도효응，시구유발전잠력적항종류약물。
The purpose of the test was to investigate the effects of MENK on cell proliferafion and apotosis in MSB-1 cells. The cultured MSB-1 cells were treated with MENK.Optical micro?scope was used to observe the morphology, the proliferation of MSB-1 cells was detected by MTT method.Cell apeptosis was detected by flow cytometry. MSB-1 cells gradually became blunt, or even shrinked treated with 10-12 mol/l MENK. MENK inhibited the proliferation of MSB-1 cells compared with control group, the cell growth inhibition rate was 19%~46%(P<0.01), and the apoptosis rate was(62.04 ± 3.32)%(P<0.01). MENK induced the apoptosis and in?hibited the cell proliferation of MSB-1, which was a potential antitumor drugs.