当代医学
噹代醫學
당대의학
CHINA CONTEMPORARY MEDICINE
2013年
19期
23-24,25
,共3页
结肠炎%溃疡性%白细胞介素-13%白细胞介素-17%免疫组化%酶联免疫吸附测定
結腸炎%潰瘍性%白細胞介素-13%白細胞介素-17%免疫組化%酶聯免疫吸附測定
결장염%궤양성%백세포개소-13%백세포개소-17%면역조화%매련면역흡부측정
Colitis%Ulcerative%Interleukin-13%Interleukin-17%Immunohistochemistry%ELISA
目的探讨IL-13、IL-17在溃疡性结肠炎（ulcerative colitis，UC）患者结肠粘膜和血清中的表达水平及意义。方法收集30例经内镜检查证实为UC患者的结肠粘膜和血清抗体，10例性别、年龄相匹配的结肠息肉患者作为对照组。用免疫组化法和酶联免疫吸附法（ELISA）检测IL-13及IL-17的表达及其血清含量。结果 UC组患者结肠粘膜阳性细胞数和血清中IL-17的浓度明显高于对照组，差异有统计学意义(P<0.01)；UC组患者IL-13结肠粘膜的阳性细胞数和血清中浓度低于对照组，差异有统计学意义(P<O.05)；在轻度、中度、重度UC患者结肠粘膜阳性细胞数和血清中IL-17的浓度呈逐渐升高趋势；而UC组患者IL-13结肠粘膜阳性细胞数和血清中IL-13的浓度呈下降趋势；2组间比较均存在显著差异(P<0.05)。Pearson相关分析发现，UC组结肠粘膜阳性细胞数与血清中IL-13和IL-17浓度呈负相关（P<0.05）。结论 IL-13、IL-17参与UC的炎症过程；两者表达水平有密切相关性；IL-13与IL-17的平衡失调可能是导致UC形成的一个重要原因。
目的探討IL-13、IL-17在潰瘍性結腸炎（ulcerative colitis，UC）患者結腸粘膜和血清中的錶達水平及意義。方法收集30例經內鏡檢查證實為UC患者的結腸粘膜和血清抗體，10例性彆、年齡相匹配的結腸息肉患者作為對照組。用免疫組化法和酶聯免疫吸附法（ELISA）檢測IL-13及IL-17的錶達及其血清含量。結果 UC組患者結腸粘膜暘性細胞數和血清中IL-17的濃度明顯高于對照組，差異有統計學意義(P<0.01)；UC組患者IL-13結腸粘膜的暘性細胞數和血清中濃度低于對照組，差異有統計學意義(P<O.05)；在輕度、中度、重度UC患者結腸粘膜暘性細胞數和血清中IL-17的濃度呈逐漸升高趨勢；而UC組患者IL-13結腸粘膜暘性細胞數和血清中IL-13的濃度呈下降趨勢；2組間比較均存在顯著差異(P<0.05)。Pearson相關分析髮現，UC組結腸粘膜暘性細胞數與血清中IL-13和IL-17濃度呈負相關（P<0.05）。結論 IL-13、IL-17參與UC的炎癥過程；兩者錶達水平有密切相關性；IL-13與IL-17的平衡失調可能是導緻UC形成的一箇重要原因。
목적탐토IL-13、IL-17재궤양성결장염（ulcerative colitis，UC）환자결장점막화혈청중적표체수평급의의。방법수집30례경내경검사증실위UC환자적결장점막화혈청항체，10례성별、년령상필배적결장식육환자작위대조조。용면역조화법화매련면역흡부법（ELISA）검측IL-13급IL-17적표체급기혈청함량。결과 UC조환자결장점막양성세포수화혈청중IL-17적농도명현고우대조조，차이유통계학의의(P<0.01)；UC조환자IL-13결장점막적양성세포수화혈청중농도저우대조조，차이유통계학의의(P<O.05)；재경도、중도、중도UC환자결장점막양성세포수화혈청중IL-17적농도정축점승고추세；이UC조환자IL-13결장점막양성세포수화혈청중IL-13적농도정하강추세；2조간비교균존재현저차이(P<0.05)。Pearson상관분석발현，UC조결장점막양성세포수여혈청중IL-13화IL-17농도정부상관（P<0.05）。결론 IL-13、IL-17삼여UC적염증과정；량자표체수평유밀절상관성；IL-13여IL-17적평형실조가능시도치UC형성적일개중요원인。
Objective The study aims to investigate the levels IL-13 and IL-17 in colonic mucosa and serum of patients with ulcerative colitis (UC). Methods Antibodies in colonicmocosa and serum were harvested from 30 UC subjects determined by colonoscope. 10 subjects with colonic polyps of the same age and gender were selected as controls. Immunohistochemistry and ELISA were used to evaluate levels of IL-13 and IL-17 in colonic mucosa and serum. Results Concentrations and positive cells of IL-17 were significantly higher in UC patients than that in controls (P﹤0.01) and levels and positive cells of IL-13 significantly decreased in UC group compared with control group (P<0.05). Besides, levels of IL-17 increased progressively in UC of mild, moderate and severe type and levels of IL-13 exhibited an decline change. The difference between groups were significant (P﹤0.05). Pearson Correlation Analysis showed a negative association of serum levels and positive cells between IL-13 and IL-17 (P﹤0.05). Conclusion IL-13 and IL-17 are closely related in UC and play an important role in the inflammatory progress of this disease.
