安徽卫生职业技术学院学报
安徽衛生職業技術學院學報
안휘위생직업기술학원학보
JOURNAL OF ANHUI HEALTH VOCATIONAL & TECHNICAL COLLEGE
2014年
2期
95-95,96
,共2页
荧光定量PCR%HBV-DNA含量%乙肝血清标志物%ELISA
熒光定量PCR%HBV-DNA含量%乙肝血清標誌物%ELISA
형광정량PCR%HBV-DNA함량%을간혈청표지물%ELISA
Fluorescence quantitative PCR%HBV-DNA content%Hepatitis B serum marker%ELISA
目的:探讨ELISA检测乙肝病毒血清标志物与荧光定量PCR检测HBV-DNA结果的相关性。方法:采用ELISA方法检测285例患者乙肝病毒血清标志物(HBV-M),同时用荧光定量PCR检测其HBV-DNA含量。结果:139例HBsAg (+)、HBeAg(+)、抗-HBc(+)患者中有124例患者阳性(血清HBV-DNA含量≥103copy/ml为阳性),阳性率为89.2%;92例HBsAg(+)、HBeAb(+)、抗-HBc(+)患者中有43例阳性,阳性率为46.7%;27例HBsAg(+)、抗-HBc(+)患者中有10例性,阳性率为37%;10例HBsAg(+)、HBeAg(+)患者中有9例阳性,阳性率为90%;11例HBsAg(+)、HBeAg(±)、抗-HBc(+)患者中有9例阳性,阳性率81.8%,6例单独HBsAg(+)患者中有0例阳性,阳性率为0。结论:所有分组中HBeAg(+)组病毒复制水平最高,其与HBV-DNA含量密切相关;抗-HBe(+)、抗-HBc(+)患者乙肝病毒复制并非完全停止,只是其复制水平明显降低,荧光定量PCR检测HBV-DNA含量可表达HBV感染和病毒复制水平。
目的:探討ELISA檢測乙肝病毒血清標誌物與熒光定量PCR檢測HBV-DNA結果的相關性。方法:採用ELISA方法檢測285例患者乙肝病毒血清標誌物(HBV-M),同時用熒光定量PCR檢測其HBV-DNA含量。結果:139例HBsAg (+)、HBeAg(+)、抗-HBc(+)患者中有124例患者暘性(血清HBV-DNA含量≥103copy/ml為暘性),暘性率為89.2%;92例HBsAg(+)、HBeAb(+)、抗-HBc(+)患者中有43例暘性,暘性率為46.7%;27例HBsAg(+)、抗-HBc(+)患者中有10例性,暘性率為37%;10例HBsAg(+)、HBeAg(+)患者中有9例暘性,暘性率為90%;11例HBsAg(+)、HBeAg(±)、抗-HBc(+)患者中有9例暘性,暘性率81.8%,6例單獨HBsAg(+)患者中有0例暘性,暘性率為0。結論:所有分組中HBeAg(+)組病毒複製水平最高,其與HBV-DNA含量密切相關;抗-HBe(+)、抗-HBc(+)患者乙肝病毒複製併非完全停止,隻是其複製水平明顯降低,熒光定量PCR檢測HBV-DNA含量可錶達HBV感染和病毒複製水平。
목적:탐토ELISA검측을간병독혈청표지물여형광정량PCR검측HBV-DNA결과적상관성。방법:채용ELISA방법검측285례환자을간병독혈청표지물(HBV-M),동시용형광정량PCR검측기HBV-DNA함량。결과:139례HBsAg (+)、HBeAg(+)、항-HBc(+)환자중유124례환자양성(혈청HBV-DNA함량≥103copy/ml위양성),양성솔위89.2%;92례HBsAg(+)、HBeAb(+)、항-HBc(+)환자중유43례양성,양성솔위46.7%;27례HBsAg(+)、항-HBc(+)환자중유10례성,양성솔위37%;10례HBsAg(+)、HBeAg(+)환자중유9례양성,양성솔위90%;11례HBsAg(+)、HBeAg(±)、항-HBc(+)환자중유9례양성,양성솔81.8%,6례단독HBsAg(+)환자중유0례양성,양성솔위0。결론:소유분조중HBeAg(+)조병독복제수평최고,기여HBV-DNA함량밀절상관;항-HBe(+)、항-HBc(+)환자을간병독복제병비완전정지,지시기복제수평명현강저,형광정량PCR검측HBV-DNA함량가표체HBV감염화병독복제수평。
Objective:ELISA to detect hepatitis B virus serum markers and the correlation of fluorescent quantitative PCR to detect HBV-DNA results.Method:By ELISA method to detect our hospital 285 cases of patients with hepatitis B virus serum marker (HBV-M), at the same time by fluorescence quantitative PCR to detect the HBV-DNA content.Result:139 cases of HBsAg (+) HBeAg (+)-resistant HBc (+) in patients with 124 cases of patients with positive serum HBV-DNA content 103 copy/ml is positive), the positive rate of 89.2%;92 cases of HBsAg (+) HBeAb (+)-resistant HBc (+) in 43 patients with positive, positive rate is 46.7%;27 cases of HBsAg (+) Anti-HBc (+) in 10 patients with positive, positive rate is 37%;10 cases of HBsAg (+) HBeAg (+) 9 cases of patients with positive, positive rate is 90%;11 cases of HBsAg (+) HBeAg (±) anti-HBc (+) 9 cases of patients with positive, positive rate was 81.8%, 6 cases of HBsAg (+) alone have 0 in patients with positive, positive rate is 0.Conclusion:All groups in HBeAg (+) group of viral replication, the highest level it is closely related to HBV-DNA content;Anti-HBe (+) Anti-HBc(+) in patients with hepatitis B virus replication is not a full stop, just copy it level significantly decreased, fluorescence quantitative PCR to detect HBV-DNA content can express HBV infection and virus replication level.