CAJ | 학술논문

利用真空高温退火技术制备不同退火温度的Ti-O粉末、X线衍射仪、Zeta电位和比表面积等表征材料，采用Bradford法测纤维蛋白原在其表面的吸附解吸附行为，运用红外线光谱分析纤维蛋白原在其表面吸附后二级结构的变化，用流式细胞仪研究其对血小板的激活程度。研究表明，800℃高温退火使得Ti-O粉末由锐钛矿转化为金红石，比表面积减少10倍，携带有更多的负电荷；吸附血浆纤维蛋白原的数量减少到67μg，且维持原有的二级结构，并存在较大的解吸附；不易引起血小板的激活，具有更好的血液相容性。
이용진공고온퇴화기술제비불동퇴화온도적Ti-O분말、X선연사의、Zeta전위화비표면적등표정재료，채용Bradford법측섬유단백원재기표면적흡부해흡부행위，운용홍외선광보분석섬유단백원재기표면흡부후이급결구적변화，용류식세포의연구기대혈소판적격활정도。연구표명，800℃고온퇴화사득Ti-O분말유예태광전화위금홍석，비표면적감소10배，휴대유경다적부전하；흡부혈장섬유단백원적수량감소도67μg，차유지원유적이급결구，병존재교대적해흡부；불역인기혈소판적격활，구유경호적혈액상용성。
Vacuum annealing was used to prepare the Ti-O powder at different annealing temperatures. The structure and surface properties were characterized by X-ray diffraction,Zeta potential test and surface area measurement respectively. Bradford method was applied to determine the adsorption and desorption of fibrinogen on the powder,and Fourier transform infrared spectroscopy was used to analyze the change of secondary structure of adsorbed fibrinogen on the samples. Then flow cytometry was used to measure the degree of platelets activation. The results show that after 800 ℃annealing process,the crystal structure of Ti-O powder transformed from anatase to rutile with 10 times less total surface area and more negative charges,and the amount of fibrinogen adsorption was decreased to 67μg and retained its normal conformation. Less platelet was activated by the annealed Ti-O powder,which proves that it has an excellent blood-compatibility.