CAJ | 학술논문

目的：研究C-fos及Reg-Ⅲβ在大鼠背根反射介导的神经病理痛中的表达变化；并探讨尼莫地平治疗对其的影响。方法建立SD大鼠选择性坐骨神经损伤（ SNI）模型，采用Von Frey 纤维丝测量不同时间点手术侧后肢的机械痛阈。采用实时荧光定量聚合酶链式反应（ RT-qPCR ）检测坐骨神经损伤后不同时间内Reg-Ⅲβ及C-fos的mRNA表达，以及不同剂量尼莫地平治疗对这些分子表达的影响。结果与-1 d相比，1、3、5、7、14 d大鼠其SNI术后各时间段脊髓背角组织中的Reg-Ⅲβ及C-fos mRNA表达均普遍上调。腹腔注射尼莫地平对Reg-ⅢβmRNA的表达上调产生明显的时间和剂量依赖性的抑制作用。模型组大鼠的机械痛阈在SNI 术后1 d即明显低于基础痛阈，术后5d降至最低，随后缓慢恢复。尼莫地平治疗后大鼠机械痛阈仍呈下降趋势，但其幅度较模型组显著减小，且术后转归时间显著提前。这些治疗效果随药物浓度提升而明显加大。结论选择性坐骨神经损伤可引起大鼠脊髓背角组织中C-fos及Reg-ⅢβmRNA表达发生时序性变化，尼莫地平能显著减轻神经病理性痛并缩短疼痛转归时间，其机制可能与逆转背根神经节介导的脊髓背角中C-fos及Reg-Ⅲβ的病理性表达有关。
목적：연구C-fos급Reg-Ⅲβ재대서배근반사개도적신경병리통중적표체변화；병탐토니막지평치료대기적영향。방법건립SD대서선택성좌골신경손상（ SNI）모형，채용Von Frey 섬유사측량불동시간점수술측후지적궤계통역。채용실시형광정량취합매련식반응（ RT-qPCR ）검측좌골신경손상후불동시간내Reg-Ⅲβ급C-fos적mRNA표체，이급불동제량니막지평치료대저사분자표체적영향。결과여-1 d상비，1、3、5、7、14 d대서기SNI술후각시간단척수배각조직중적Reg-Ⅲβ급C-fos mRNA표체균보편상조。복강주사니막지평대Reg-ⅢβmRNA적표체상조산생명현적시간화제량의뢰성적억제작용。모형조대서적궤계통역재SNI 술후1 d즉명현저우기출통역，술후5d강지최저，수후완만회복。니막지평치료후대서궤계통역잉정하강추세，단기폭도교모형조현저감소，차술후전귀시간현저제전。저사치료효과수약물농도제승이명현가대。결론선택성좌골신경손상가인기대서척수배각조직중C-fos급Reg-ⅢβmRNA표체발생시서성변화，니막지평능현저감경신경병이성통병축단동통전귀시간，기궤제가능여역전배근신경절개도적척수배각중C-fos급Reg-Ⅲβ적병이성표체유관。
Objective To explore the expressions of C-fos and Reg-Ⅲβin the dorsal root of rats with neuropathic pain mediated by dorsal root reflex , and to investigate the effect of nimodipine treatment on them .Methods After the establishment of dorsal root reflex-mediated neuropathic pain by selective spared nerve injury ( SNI) in SD rats, Von Frey filament measurement and real-time fluorescence quantitative polymerase chain reaction ( RT-qPCR ) were performed to determine the mechanical pain threshold of rats ’ hind limbs and the endogenous mRNA expressions of C-fos and Reg-Ⅲβin the dorsal root at different time points , respectively .Nimodipine was given at gradient doses in the experimental rats to examine its effect on the molecular expressions .Results Compared with the unchanged mRNA expressions of C-fos and Reg-Ⅲβin rats from shamed-surgery group , they uniformly increased in rats from SNI groups .After intraperitoneal injection of nimodipine , the increasing tendencies of both C-fos and Reg-Ⅲβexpression were substantially inhibited in a time-and dose-dependent manner .Similar antagonistic effect of nimodipine treatment with regard to mechanical pain threshold was also determined in a dose-dependent manner , which progressively decreased in the SNI model rats as early as 1 day after .surgery compared to the basal level .It continued till 5 days after surgery and followed by a slow recovery .On day 5 after surgery , nimodipine treatment resulted in a significant inhibition on the decline of pain threshold .Conclusions Selective SNI substantially induces mRNA expressions of C-fos and Reg-Ⅲβin the dorsal root of rats .Nimodipine treatment can significantly alleviate the neuropathic pain and reduce its duration , which may be potentially related to the reversal of C-fos and Reg-Ⅲβexpressions .