临床神经外科杂志
臨床神經外科雜誌
림상신경외과잡지
JOURNAL OF CLINICAL NEUROSURGERY
2014年
2期
94-95,98
,共3页
脑胶质瘤%端粒结合蛋白-1%免疫组织化学
腦膠質瘤%耑粒結閤蛋白-1%免疫組織化學
뇌효질류%단립결합단백-1%면역조직화학
gliomas%telomeric repeat binding factor-1%immunohistochemical
目的：探讨端粒结合蛋白-1（TRF-1）在不同级别脑胶质瘤及正常脑组织中的表达及其临床意义。方法选取58例脑胶质瘤和10例脑外伤内减压脑组织石蜡切片标本作为研究对象，应用免疫组化技术检测各组TRF1的表达水平，应用半定量法计算不同标本肿瘤细胞免疫标记的频率和强度积分。结果共58例不同级别胶质细胞瘤，其中低度恶性组（WHOⅠ、Ⅱ级）27例，高度恶性组（WHOⅢ、Ⅳ级）31例，10例脑外伤病人内减压脑组织作为正常对照。在所有胶质瘤组织及正常脑组织中均检测到 TRF1表达，其中正常脑组织中TRF1表达高积分构成比90．00％，低度恶性胶质瘤组（WHOⅠ、Ⅱ级）的TRF1表达高积分构成比62．96％，高度恶性胶质瘤组（WHOⅢ、Ⅳ级）29．03％。统计分析发现，TRF1在不同级别胶质瘤中的表达与其恶性程度成负相关。结论在人脑胶质瘤和正常脑组织均有TRF1的广泛表达，其在不同级别脑胶质瘤中的表达水平与其恶性程度负相关，即随肿瘤的恶性程度的增高出现表达下调，TRF1可作为脑胶质瘤临床病理分级和恶性程度判断的参考指标。
目的：探討耑粒結閤蛋白-1（TRF-1）在不同級彆腦膠質瘤及正常腦組織中的錶達及其臨床意義。方法選取58例腦膠質瘤和10例腦外傷內減壓腦組織石蠟切片標本作為研究對象，應用免疫組化技術檢測各組TRF1的錶達水平，應用半定量法計算不同標本腫瘤細胞免疫標記的頻率和彊度積分。結果共58例不同級彆膠質細胞瘤，其中低度噁性組（WHOⅠ、Ⅱ級）27例，高度噁性組（WHOⅢ、Ⅳ級）31例，10例腦外傷病人內減壓腦組織作為正常對照。在所有膠質瘤組織及正常腦組織中均檢測到 TRF1錶達，其中正常腦組織中TRF1錶達高積分構成比90．00％，低度噁性膠質瘤組（WHOⅠ、Ⅱ級）的TRF1錶達高積分構成比62．96％，高度噁性膠質瘤組（WHOⅢ、Ⅳ級）29．03％。統計分析髮現，TRF1在不同級彆膠質瘤中的錶達與其噁性程度成負相關。結論在人腦膠質瘤和正常腦組織均有TRF1的廣汎錶達，其在不同級彆腦膠質瘤中的錶達水平與其噁性程度負相關，即隨腫瘤的噁性程度的增高齣現錶達下調，TRF1可作為腦膠質瘤臨床病理分級和噁性程度判斷的參攷指標。
목적：탐토단립결합단백-1（TRF-1）재불동급별뇌효질류급정상뇌조직중적표체급기림상의의。방법선취58례뇌효질류화10례뇌외상내감압뇌조직석사절편표본작위연구대상，응용면역조화기술검측각조TRF1적표체수평，응용반정량법계산불동표본종류세포면역표기적빈솔화강도적분。결과공58례불동급별효질세포류，기중저도악성조（WHOⅠ、Ⅱ급）27례，고도악성조（WHOⅢ、Ⅳ급）31례，10례뇌외상병인내감압뇌조직작위정상대조。재소유효질류조직급정상뇌조직중균검측도 TRF1표체，기중정상뇌조직중TRF1표체고적분구성비90．00％，저도악성효질류조（WHOⅠ、Ⅱ급）적TRF1표체고적분구성비62．96％，고도악성효질류조（WHOⅢ、Ⅳ급）29．03％。통계분석발현，TRF1재불동급별효질류중적표체여기악성정도성부상관。결론재인뇌효질류화정상뇌조직균유TRF1적엄범표체，기재불동급별뇌효질류중적표체수평여기악성정도부상관，즉수종류적악성정도적증고출현표체하조，TRF1가작위뇌효질류림상병리분급화악성정도판단적삼고지표。
Objective To explore the expression of telomeric repeat binding factor-1(TRF1) in human gliomas and normal brain tissue and its clinical significances .Methods TRF1 were detected in 58 surgical specimens of gliomas and 10 normal brain tissue by immuno-histochemical technique . Immunohistochemical scores were semi-quantified by the sum of the frequency and intensity .Results Of 58 patients with gliomas,27 were low-grade(gradeⅠ~Ⅱ) gliomas,31 were high-grade (gradeⅢ~Ⅳ) gliomas, TRF1 were expressed in all tumor samples and normal brain tissue .The level of its expression were variable .The rate of high immunohistochemical score of TRF 1 expression in low-grade malignant gliomas , high-grade malignant gliomas ,normal brain tissue were 62 .96%,29 .03%and 90 .00%respectively .The level of TRF1 expression were inversely correlated with the malignancy of gliomas.Conclusions TRF1 was expressed in both gliomas and normal brain tissues . TRF1 expression was closely related to the malignancy grade of brain tumor , it decreased in higher grade gliomas ,and may be used as a new indicator of malignancy of gliomas and meningiomas .
