CAJ | 학술논문

目的：探讨体内miR-125 b表达对人脑胶质瘤细胞侵袭力的影响及其可能的机制。方法培养原代胶质瘤细胞，分别转染miR-125b mimics和miR-125b inhibitor 慢病毒载体，上调或下调细胞中miR-125b表达水平，种植乳鼠皮下，Transwell试验评价胶质瘤细胞侵袭能力的变化，Western Blot 验证侵袭相关基质金属蛋白酶（MMP）-2、MMP-9及RECK和TIMP3蛋白的表达。结果转染miR-125b mimics能有效提高原代胶质瘤细胞中miR-125b的表达，miR-125b inhibitor 有效降低miR-125b 的表达；体内实验表明miR-125b mimics及inhibitor对胶质瘤侵袭能力无明显影响，不影响侵袭相关MMP-2、MMP-9及RECK、TIMP3蛋白的表达。结论体内miR-125b表达水平与原代胶质瘤细胞的侵袭能力无明显相关。
목적：탐토체내miR-125 b표체대인뇌효질류세포침습력적영향급기가능적궤제。방법배양원대효질류세포，분별전염miR-125b mimics화miR-125b inhibitor 만병독재체，상조혹하조세포중miR-125b표체수평，충식유서피하，Transwell시험평개효질류세포침습능력적변화，Western Blot 험증침습상관기질금속단백매（MMP）-2、MMP-9급RECK화TIMP3단백적표체。결과전염miR-125b mimics능유효제고원대효질류세포중miR-125b적표체，miR-125b inhibitor 유효강저miR-125b 적표체；체내실험표명miR-125b mimics급inhibitor대효질류침습능력무명현영향，불영향침습상관MMP-2、MMP-9급RECK、TIMP3단백적표체。결론체내miR-125b표체수평여원대효질류세포적침습능력무명현상관。
Objective To investigate the function and possible mechanisms of miR-125 b expression on the invasion of glioma in vivo .Methods Primary glioblastoma cells were separated and cultured , which were transfected with lentiviral vectors for miR-21 mimics or miR-21 inhibitors for upregulation or downregulation of miR-125b expression levels.After that, cells were directly injected subcutaneously into the flanks of nude mice .The invasive effects of primary glioblastoma cells and stem cells were measured by Transwell assay , invasion related genes were screened by mRNA array, and the expressing levels of matrix metalloprotease ( MMP)-2, MMP-9, RECK and TIMP3 were identified by Western blot .Results miR-125 b mimics transfection in vitro could effectively improve the expression of miR-125 b in primary glioblastoma cells and stem cells , miR-125 b inhibitor transfection could effectively inhibit the expression of miR-125 b.Results showed that miR-125 b mimics or inhibitor had no effects on the invasion of primary glioblastoma cells in vivo , and did not affect the expression of MMP-2, MMP-9, RECK and TIMP3.Conclusion miR-125b expression is not related to the invasion properties of primary glioblastoma cells in vivo .