CAJ | 학술논문

　　目的：观察电针对哮喘豚鼠气道重构模型气道病理结构的影响。方法：以10％卵清蛋白注射液腹腔注射致敏，第15 d 起隔天以1％卵蛋白溶液超声雾化以激发（共6周）进行造模。采用电针背三针（大杼、风门、肺俞）干预治疗，隔天1次，共6周。治疗结束时，在光学显微镜下观察各组豚鼠肺组织和支气管的病理形态学改变、嗜酸粒细胞计数，并进行病理形态学测量。结果：模型对照组出现了哮喘气道重构的病理学改变，地塞米松治疗组和电针治疗组的肺组织也有不同程度的病变表现，但明显轻于模型对照组。模型对照组的支气管及肺组织中嗜酸性粒细胞数高于正常组（P ＜0．01），电针治疗组和地塞米松治疗组均低于模型对照组（P ＜0．01）；与地塞米松治疗组相比较，电针治疗组嗜酸性粒细胞数略高（P ＜0．05）。模型对照组气道纤维组织厚度、气道内膜厚度高于正常组，电针治疗组和地塞米松治疗组都比模型对照组低（P ＜0．05）；在小气道中，模型对照组气道平滑肌厚度高于正常组，电针治疗组和地塞米松治疗组均低于模型对照组（P ＜0．05）；电针治疗组和地塞米松治疗组的观察指标无显著性差异（P ＞0．05）。结论：电针豚鼠背三针能降低气道重构豚鼠模型支气管及肺组织中嗜酸性粒细胞数，减轻气道炎症反应；降低豚鼠大气道的气道纤维组织厚度、气道内膜厚度及小气道平滑肌厚度。
　　목적：관찰전침대효천돈서기도중구모형기도병리결구적영향。방법：이10％란청단백주사액복강주사치민，제15 d 기격천이1％란단백용액초성무화이격발（공6주）진행조모。채용전침배삼침（대저、풍문、폐유）간예치료，격천1차，공6주。치료결속시，재광학현미경하관찰각조돈서폐조직화지기관적병리형태학개변、기산립세포계수，병진행병리형태학측량。결과：모형대조조출현료효천기도중구적병이학개변，지새미송치료조화전침치료조적폐조직야유불동정도적병변표현，단명현경우모형대조조。모형대조조적지기관급폐조직중기산성립세포수고우정상조（P ＜0．01），전침치료조화지새미송치료조균저우모형대조조（P ＜0．01）；여지새미송치료조상비교，전침치료조기산성립세포수략고（P ＜0．05）。모형대조조기도섬유조직후도、기도내막후도고우정상조，전침치료조화지새미송치료조도비모형대조조저（P ＜0．05）；재소기도중，모형대조조기도평활기후도고우정상조，전침치료조화지새미송치료조균저우모형대조조（P ＜0．05）；전침치료조화지새미송치료조적관찰지표무현저성차이（P ＞0．05）。결론：전침돈서배삼침능강저기도중구돈서모형지기관급폐조직중기산성립세포수，감경기도염증반응；강저돈서대기도적기도섬유조직후도、기도내막후도급소기도평활기후도。
Objective: To observe the effect of electroacupuncture (EA) on airway pathological changes in asthmatic Guinea pigs with airway remodeling. Methods: The Guinea pig model was established by sensitization with intraperitoneal injection of 10% ovalbumin (OVA) in the first day and challenge with nebulized inhalation of 1%OVA solution in the 15th day, every other day for 8 weeks. Every time before challenge,the interventive treatment of EA of back six points (Dazhu, Fengmen, Feishu) was carried out. After treatment,the pathological changes, eosinophils (EOS) count and morphometric analysis in bronchi and lung were observed under light microscope. Results: The pathological changes of airway remodeling occurred in the model group and the pathological changes in dexamethasone group and EA group were much relieved. The result of eosinophils (EOS) count showed that the number of EOS in the model group was much higher that in the normal group (P<0.01), and decreased in dexamethasone group and EA group as compared with the model group (P<0.01);as compared with that in dexamethasone group, EOS count in EA group was higher (P<0.05). The results of morphometric analysis of bronchi and lung showed that the thickness of airway intima and thickness of fiber structure in main airway of the model group was higher than those in the normal group (P<0.05),and were decreased in dexamethasone group and EA group as compared with the model group (P<0.05).In small airway, the thickness of smooth muscle in the normal group was <br> higher than those in the normal group (P<0.05), and were decreased in dexamethasone group and EA group as compared with the model group (P<0.05). The difference of the above three parameters was insignificant between dexamethasone group and EA group (P>0.05). Conclusion: The interventive treatment of EA of back six points can reduce EOS count in bronchi and lung of asthmatic guinea pigs, relieve the inflammatory reaction, decrease the thickness of airway intima and thickness of fiber structure in main airway and the thickness of smooth muscle in small airway to relieve the damage of ultrastructure.