天津农学院学报
天津農學院學報
천진농학원학보
JOURNAL OF TIANJIN AGRICULTURAL COLLEGE
2013年
2期
18-21
,共4页
柴慈江%史燕山%骆建霞%卢兴霞%武旭娜
柴慈江%史燕山%駱建霞%盧興霞%武旭娜
시자강%사연산%락건하%로흥하%무욱나
栒子%试管苗%土壤支撑%培养基%生根培养
栒子%試管苗%土壤支撐%培養基%生根培養
순자%시관묘%토양지탱%배양기%생근배양
Cotoneaster hjelmqvistii%plantlet in vitro%soil supporting%culture medium%rooting culture
以沙壤土和粘壤土分别做培养基支撑物进行栒子试管苗生根培养,试管苗的生根率、根长均明显高于琼脂支撑培养对照组。在沙壤土或粘壤土支撑培养条件下,培养基中不添加大量元素,可以显著提高栒子试管苗的生根率、根长等指标。以粘壤土做培养基支撑物,培养基中不添加微量元素和有机物,与添加者相比,生根率及根长无明显差异;培养基中添加蔗糖后,其生根率、根长等指标均明显高于未加蔗糖的处理,且培养基含蔗糖为1.5%的处理试管苗生根率达到93.3%,并显著高于含蔗糖为0.75%的处理。该研究结果可降低栒子试管苗成本,并为简化其移栽程序奠定基础。
以沙壤土和粘壤土分彆做培養基支撐物進行栒子試管苗生根培養,試管苗的生根率、根長均明顯高于瓊脂支撐培養對照組。在沙壤土或粘壤土支撐培養條件下,培養基中不添加大量元素,可以顯著提高栒子試管苗的生根率、根長等指標。以粘壤土做培養基支撐物,培養基中不添加微量元素和有機物,與添加者相比,生根率及根長無明顯差異;培養基中添加蔗糖後,其生根率、根長等指標均明顯高于未加蔗糖的處理,且培養基含蔗糖為1.5%的處理試管苗生根率達到93.3%,併顯著高于含蔗糖為0.75%的處理。該研究結果可降低栒子試管苗成本,併為簡化其移栽程序奠定基礎。
이사양토화점양토분별주배양기지탱물진행순자시관묘생근배양,시관묘적생근솔、근장균명현고우경지지탱배양대조조。재사양토혹점양토지탱배양조건하,배양기중불첨가대량원소,가이현저제고순자시관묘적생근솔、근장등지표。이점양토주배양기지탱물,배양기중불첨가미량원소화유궤물,여첨가자상비,생근솔급근장무명현차이;배양기중첨가자당후,기생근솔、근장등지표균명현고우미가자당적처리,차배양기함자당위1.5%적처리시관묘생근솔체도93.3%,병현저고우함자당위0.75%적처리。해연구결과가강저순자시관묘성본,병위간화기이재정서전정기출。
The stem sections of Cotoneaster hjelmqvistii plantlets in vitro were cultured in the medium supported by sandy loam soil and clay loam soil respectively for rooting, and the rooting rate and the root length of the plantlet cultured in the soil supporting medium were obviously higher than those of the plantlet cultured in the medium supported by agar. When cultured in the medium supported by the soil, the rooting rate and the root length of the plantlet in the medium without adding macro-elements were obviously higher than those in the medium with macro-elements added. In the medium supported by the clay loam soil, the rooting rate and the root length of the plantlet cultured in the medium without micro-elements and organic materials were obviously higher than those in the medium with micro-elements and organic materials added. Besides, in the medium supported by the clay loam soil, the rooting rate and the root length of the plantlet in the medium with sugar added were obviously higher than those of the plantlets cultured in the medium without sugar, and the rooting rate of the plantlet in the medium with 1.5%sugar was 93.3%, which were obviously higher than that of plantlet in the medium with 0.75%sugar. These results can reduce the cost and lay a foundation for simplifying the transplanting procedure of the Cotoneaster hjelmqvistii plantlets in vitro.