CAJ | 학술논문

　　目的观察银杏叶提取物对放射性中耳炎动物模型早期中耳放射性损伤的防护作用。方法44只健康豚鼠，随机分为模型对照1天组、模型对照30天组、银杏叶提取物治疗1天组、银杏叶提取物治疗30天组、空白对照组。以医用直线加速器6MV-X射线照射豚鼠左耳，3.0Gy/次，每周5次，总剂量45Gy。加用银杏叶提取物治疗的豚鼠每次照射前注射EGB0.4ml/kg，模型对照组注射同体积生理盐水，空白对照组不作处理。分别于照射剂量达45Gy后第2天和第30天处死动物，采集标本，行光镜及扫描电镜检查，比较各组豚鼠照射侧中耳黏膜病理改变和超微结构变化。结果射线剂量达45Gy后第2天，见中耳黏膜上皮增厚，炎性细胞浸润，纤毛倒伏、融合，渗出增多；照射后第30天，见中耳黏膜上皮纤毛脱落、减少，损伤加重；在照射前应用EGB的豚鼠，显示中耳黏膜损伤减轻；未用药组较用药组中耳黏膜厚度及白细胞浸润数均增高(均P＜0.05)。结论放射线可致中耳黏膜黏液纤毛输送系统功能受损，并随时间推移而加重；银杏提取物可以减轻中耳黏膜的早期放射损伤，并随时间的延长而有进一步的改善。
　　목적관찰은행협제취물대방사성중이염동물모형조기중이방사성손상적방호작용。방법44지건강돈서，수궤분위모형대조1천조、모형대조30천조、은행협제취물치료1천조、은행협제취물치료30천조、공백대조조。이의용직선가속기6MV-X사선조사돈서좌이，3.0Gy/차，매주5차，총제량45Gy。가용은행협제취물치료적돈서매차조사전주사EGB0.4ml/kg，모형대조조주사동체적생리염수，공백대조조불작처리。분별우조사제량체45Gy후제2천화제30천처사동물，채집표본，행광경급소묘전경검사，비교각조돈서조사측중이점막병리개변화초미결구변화。결과사선제량체45Gy후제2천，견중이점막상피증후，염성세포침윤，섬모도복、융합，삼출증다；조사후제30천，견중이점막상피섬모탈락、감소，손상가중；재조사전응용EGB적돈서，현시중이점막손상감경；미용약조교용약조중이점막후도급백세포침윤수균증고(균P＜0.05)。결론방사선가치중이점막점액섬모수송계통공능수손，병수시간추이이가중；은행제취물가이감경중이점막적조기방사손상，병수시간적연장이유진일보적개선。
Objective To investigate the therapeutic effect of Ginkgo Biloba extract (GBE) on the earlier mucosa damage in middle ear induced by radiation among animal models. Methods Forty-four adult guinea pigs were randomly divided into 5 groups, i.e. model control group 1 (tested on the 2nd day following radiotherapy ended), model control group 2 (tested on the 30th day following radiotherapy ended), EGB treating group 1 (tested on the 2nd day following radiotherapy ended), EGB treating group 2 (tested on the 30th day following radiotherapy ended) and blank control group. Animals in the first four groups were exposed to X-rays radiation at a total dosage of 45 Gy, which was administered to the left ear of each guinea pig at 3.0 Gy each time and 5 times per week, by use of a linear accelerator 6MV-X. Animals in EGB treating groups were treated with 0.4 ml/kg EGB injected intra-peritoneally 30 min before radiation each time, while those in model control groups were injected intra-peritoneally with normal saline at the same volume in the same way. Healthy guinea pigs in blank control group were given no any treatment. Tissue samples were taken from the tympanic bullas of animals put into death on the second day and thirtieth day respectively when the radiation period ended for light microscopy and scanning electron microscopy to observe such indexes as mucosa thickness, leukocyte infiltration and the morphology of cilia. Results As seen under light microscope and scanning electron microscope from the modeling animals, middle ear cleft was accumulated with effusion and its mucosa became thicker with inflammatory cells infiltrating and cilia fell off, even fused or collapsed on the 2nd day following the radiation treatment ended. Until the 30th day, such damage in middle ear mucosa became more severe. As a comparison, such damage in middle ear mucosa significantly relieved among the guinea pigs in EGB treating groups, with statistical significance in the differences of mucosa thickness and infiltrating leukocyte number in mucosa among these groups (P<0.05). Conclusion Radiation can induce damage to the middle ear mucosa leading to dysfunction of mucus-cilia transporting system here, with a tendency becoming more severe during a longer period even though the radiation being withdrawn. EGB can provide a significant protecting effect on radiation-induced earlier damage in middle ear mucosa, more effective as the time course even longer.