CAJ | 학술논문

　　本文利用生物信息学方法从转录组测序数据库中分离了苎麻纤维素合成酶CesA1（BnCesA1）基因全长编码区序列（ NCBI 登录号： KC112993），并与大麻纤维素合成酶（CsCesA1）进行了氨基酸组成成分、理化性质以及二级结构比较，构建了CesA1基因家族的进化树。结果显示BnCesA1由3249个碱基组成，编码1082个氨基酸组成的蛋白， CsCesA1由3198个碱基组成，编码1065个氨基酸组成的蛋白。它们是疏水性脂溶蛋白，都含有6个跨膜区，二级结构以α－螺旋和无规卷曲为主，存在小部分β－转角或扩展链。两基因的核酸序列同源性为86％，氨基酸序列同源性为93％。本文结果为麻类作物纤维素合成酶基因的进一步功能分析提供了基础。
　　본문이용생물신식학방법종전록조측서수거고중분리료저마섬유소합성매CesA1（BnCesA1）기인전장편마구서렬（ NCBI 등록호： KC112993），병여대마섬유소합성매（CsCesA1）진행료안기산조성성분、이화성질이급이급결구비교，구건료CesA1기인가족적진화수。결과현시BnCesA1유3249개감기조성，편마1082개안기산조성적단백， CsCesA1유3198개감기조성，편마1065개안기산조성적단백。타문시소수성지용단백，도함유6개과막구，이급결구이α－라선화무규권곡위주，존재소부분β－전각혹확전련。량기인적핵산서렬동원성위86％，안기산서렬동원성위93％。본문결과위마류작물섬유소합성매기인적진일보공능분석제공료기출。
The full length of BnCesA1 cDNA coding region was isolated from transcriptome sequen-cing data by bioinformatics method , and logined in GeneBank ( accession number:KC112993 ) .Sever-al parameters of BnCesA1 and CsCesA1 including sequences composition , physicochemical property , to-pological structure of transmembrane regions , hydrophobicity or hydrophilicity , secondary structures were predicted.The results showed that BnCesA1 ORF was 3249 bp in length , encoded a protein of 1082 amino acids;and CsCesA1 was 3198 bp, encoded a protein of 1065 amino acids.The two genes showed the similarity of 86%in nucleotides and 93%in protein, and the secondary structure was quite similar.The results of this investigation provided a significant foundation for further research on CesA genes of fi-ber crops.