CAJ | 학술논문

　　本研究通过分析苎麻转录组中的基因注释，首次筛选出8个苎麻WRKY转录因子基因序列。结合拟南芥、水稻、大豆和小麦等作物的10个已报道具有抗旱功能的WRKY转录因子序列进行聚类分析，发现这10个具有抗旱功能的WRKY基因和苎麻的8个WRKY基因的蛋白质编码序列高度保守。利用MEGA软件进一步构建这18个WRKY基因的蛋白质序列进化树，结果发现18个WRKY转录因子可以分为4组，其中Unigene5705与CL3777.Contig1和大豆的Gm-WRKY13同属一组，即WRKY－1类；而Unigene5211 CL5210.Contig1， Unigene14199和拟南芥的AtWRKY2同属WRKY－2类；两个苎麻WRKY基因（ Unigene22787和Unigene29108）则和六个已报道的抗旱WRKY基因（ AtWRKY57， OsWRKY08， OsWRKY11， OsWRKY72， GmWRKY21和GmWRKY54）同属 WRKY －3类； CL34.Contig1， TcWRKY53和 ABO3/AtWRKY63同属WRKY－4组。由于所有10个拟南芥、水稻、大豆和小麦WRKY基因具有抗旱功能，因此我们推测苎麻的这8个WRKY转录因子很可能也具有抗旱调控功能。
　　본연구통과분석저마전록조중적기인주석，수차사선출8개저마WRKY전록인자기인서렬。결합의남개、수도、대두화소맥등작물적10개이보도구유항한공능적WRKY전록인자서렬진행취류분석，발현저10개구유항한공능적WRKY기인화저마적8개WRKY기인적단백질편마서렬고도보수。이용MEGA연건진일보구건저18개WRKY기인적단백질서렬진화수，결과발현18개WRKY전록인자가이분위4조，기중Unigene5705여CL3777.Contig1화대두적Gm-WRKY13동속일조，즉WRKY－1류；이Unigene5211 CL5210.Contig1， Unigene14199화의남개적AtWRKY2동속WRKY－2류；량개저마WRKY기인（ Unigene22787화Unigene29108）칙화륙개이보도적항한WRKY기인（ AtWRKY57， OsWRKY08， OsWRKY11， OsWRKY72， GmWRKY21화GmWRKY54）동속 WRKY －3류； CL34.Contig1， TcWRKY53화 ABO3/AtWRKY63동속WRKY－4조。유우소유10개의남개、수도、대두화소맥WRKY기인구유항한공능，인차아문추측저마적저8개WRKY전록인자흔가능야구유항한조공공능。
In this research , we screened 8 ramie WRKY transcription factor genesequences for the first time by analyzing transcriptome gene annotation.10 WRKY transcription factor sequences related to the function of drought resistance have been reported in arabidopsis , rice, soybean and wheat etc.The 18 gene coding sequences were highly conserved by cluster analysis.We constructed the WRKY gene phylogenetic tree by MEGA software for these 18 WRKY genes , and found that the 18 WRKY transcrip-tion factors could be divided into four groups: Unigene5705 , CL3777.Contig1 and GmWRKY13 were classified into the WRKY -1 group; Unigene5211, CL5210.Contig1, Unigene14199 and AtWRKY2 were classified into WRKY -2 group; Unigene22787, Unigene29108, AtWRKY57, OsWRKY08, Os-WRKY11 , OsWRKY72 , GmWRKY21 and GmWRKY54 were classified into WRKY -3 group;CL34.Contig1 , TcWRKY53 and ABO3/AtWRKY63 was classified into WRKY-4 group.Since that the function of drought tolerance of 10 WRKY TFs had been characterized , we suggested that these 8 ramie WRKY TFs probably have the potential of drought -tolerance function.