CAJ | 학술논문

　　以蛋白核小球藻820为实验材料，研究了3种盐度(15、30、45)对其生长、叶绿素荧光参数和两种代谢酶活性的影响，以了解该小球藻对盐度的适应能力.结果表明，蛋白核小球藻820的生长随盐度增加而变慢；而油脂含量随盐度增加而升高.叶绿素荧光参数中的PSII最大光能转化效率(Fv/Fm)、PSII实际光能转化效率(ΦPSII)、光化学淬灭系数(qP)随盐度升高下降，而非光化学淬灭系数(NPQ)随盐度升高而上升.超氧化物歧化酶(SOD)活性变化大致趋势是低盐和高盐下活性较高，碳酸酐酶(CA)活性则随盐度升高而降低，第5 d时45盐度是30盐度培养的0.43倍.因此，认为高盐一定程度地抑制了蛋白核小球藻的生长、叶绿素荧光参数和CA活性，但是促进了总脂含量和抗氧化酶SOD活性的提高.
　　이단백핵소구조820위실험재료，연구료3충염도(15、30、45)대기생장、협록소형광삼수화량충대사매활성적영향，이료해해소구조대염도적괄응능력.결과표명，단백핵소구조820적생장수염도증가이변만；이유지함량수염도증가이승고.협록소형광삼수중적PSII최대광능전화효솔(Fv/Fm)、PSII실제광능전화효솔(ΦPSII)、광화학쉬멸계수(qP)수염도승고하강，이비광화학쉬멸계수(NPQ)수염도승고이상승.초양화물기화매(SOD)활성변화대치추세시저염화고염하활성교고，탄산항매(CA)활성칙수염도승고이강저，제5 d시45염도시30염도배양적0.43배.인차，인위고염일정정도지억제료단백핵소구조적생장、협록소형광삼수화CA활성，단시촉진료총지함량화항양화매SOD활성적제고.
Chlorella pyrenoidosa is a kind of important economic microalgae. In this paper, the effects of different salinity (15, 30 and 45) on the growth, total lipid content, chlorophyll fluorescence parameters and enzymic activities of C. pyrenoidosa 820 are investigated in order to understand how the alga responds to salinity. Results show that, with the increase of salinity, the growth speed of C. pyrenoidosa 820 decreases, but the lipid contents keep increasing. The chlorophyll fluorescence parameters of the maximal photochemical efficiency of PSII (Fv/Fm), actual photochemical efficiency of PSII (ΦPSII) and photochemical quenching (qP) declinee with the increasing salinity, whereas the non-photochemical quenching (NPQ) is enhanced with the increasing salinity. The superoxide dismutase (SOD) activities are higher under the 15 and 45 salinity than that of 30. The carbonic anhydrase (CA) activities are reversely propotional to the salinity increase, and CA activity of 45 is 43%of that of 30 salinity on the fifth day. These results suggest that the growth, chlorophyll fluorescence parameters and CA activity of C. pyrenoidosa are inhibited to a certain extent by the increased salinity, but the lipid content and SOD activity are enhanced to adapt the adverse salt environment.