CAJ | 학술논문

　　通过不同的样品处理方式、IPG缓冲液浓度、染色方法和pH等条件的对比试验，优化建立了中华鳖肌肉组织蛋白质的双向电泳技术体系.结果表明：蛋白样品经试剂盒纯化后可显著提高等电聚焦效果； IPG缓冲液浓度为0.5%的聚焦效果好于2.0%浓度；考染所获蛋白质点较少，而银染所获蛋白质点明显增多；pH 4~7胶条的等电聚焦效果明显好于pH 3~10的胶条.
　　통과불동적양품처리방식、IPG완충액농도、염색방법화pH등조건적대비시험，우화건립료중화별기육조직단백질적쌍향전영기술체계.결과표명：단백양품경시제합순화후가현저제고등전취초효과； IPG완충액농도위0.5%적취초효과호우2.0%농도；고염소획단백질점교소，이은염소획단백질점명현증다；pH 4~7효조적등전취초효과명현호우pH 3~10적효조.
A 2-DE related technique is introduced and optimized for muscle proteome of Chinese soft-shelled turtle Pelodiscus sinensis. The comparative tests are conducted between different sample treatment, concen-tration of IPG buffer, staining methods and pH of IPG strips. The result indicate that the effect of isoelectric focusing (IEF) of purified protein sample is better than that of initial extracting protein, and that the concentration of 0.5%IPG buffer is more suitable than the concentration of 2.0%. Based on stained comparison, we suggest that the silver staining can detect more protein spots than coomassie brilliant blue (CCB) staining.