CAJ | 학술논문

　　本文首次采用斑马鱼为模型动物，以糖皮质激素泼尼松龙为造模用药，建立一种快速、高效的骨质疏松新动物模型。选用受精后4 d或5 d的幼鱼暴露于不同浓度泼尼松龙溶液中，同时设0.5%DMSO溶媒阴性对照组，28.5益下在24孔板中培养5 d，至斑马鱼颅骨形成。采用茜素红对培养至9 d或10 d的斑马鱼骨骼进行染色，以显微检测、数码成像方法定量分析骨骼染色区域。与溶媒阴性对照组相比，2.5，10，25滋mol?L-1泼尼松龙组的斑马鱼骨骼染色面积和染色光密度值显著减少，提示泼尼松龙使斑马鱼骨骼矿化量和骨密度显著降低，成功诱导斑马鱼骨质疏松。并用该模型成功验证鲑鱼降钙素和依替膦酸二钠防治骨质疏松的作用。
　　본문수차채용반마어위모형동물，이당피질격소발니송룡위조모용약，건립일충쾌속、고효적골질소송신동물모형。선용수정후4 d혹5 d적유어폭로우불동농도발니송룡용액중，동시설0.5%DMSO용매음성대조조，28.5익하재24공판중배양5 d，지반마어로골형성。채용천소홍대배양지9 d혹10 d적반마어골격진행염색，이현미검측、수마성상방법정량분석골격염색구역。여용매음성대조조상비，2.5，10，25자mol?L-1발니송룡조적반마어골격염색면적화염색광밀도치현저감소，제시발니송룡사반마어골격광화량화골밀도현저강저，성공유도반마어골질소송。병용해모형성공험증해어강개소화의체련산이납방치골질소송적작용。
Zebrafish was selected as model animal, and glucocorticoid prednisolone was used as a model com-pound to develop a rapid and high efficient osteoporosis novel model. A 5-day exposure was given to a series of prednisolone solutions with a range of concentration starting from either 4 days post fertilization (dpf) or 5 dpf. And 0.5% DMSO was selected as the negative solvent control group. All groups were incubated in 24-well plates (28.5℃). Zebrafish at 9 dpf or 10 dpf were stained with alizarin red. Quantitative analysis of the stained area was performed by microscopic inspection and digital imaging methods to reflect the amount of bone mineralization. And the results showed that the prednisolone group at the concentration of 2.5 μmol?L-1, 10 μmol?L-1 and 25μmol?L-1 can obviously decrease the staining area and the staining optical density values when compared with the negative solvent control group (0.5% DMSO), which suggested that prednisolone can significantly reduce the ze-brafish mineralized bone and the bone mineral density. Therefore, osteoporosis model using zebrafish induced by prednisolone was successfully developed. In addition, zebrafish osteoporosis model was successfully used to verify the effect of salmon calcitonin and etidronate disodium in the prevention of osteoporosis.