CAJ | 학술논문

目的观察阿尔茨海默病(AD)与血管性痴呆(VaD)患者血浆中DNA氧化损伤标志物8羟基脱氧鸟苷(8-OHdG)水平的变化,探讨DNA氧化损伤在AD和VaD发病中的作用. 方法用酶联免疫吸附试验的方法测定35例AD患者和28例VaD患者以及与之年龄相匹配的23例健康者血浆中8-OHdG的水平,用简易智力量表(MMSE)评测AD和VaD患者的认知功能. 结果 AD组、VaD组、健康对照组患者血浆中8-OHdG浓度分别为(601.8±266.0)ng/L、(1748.0±665.6)ng/L和(352.0±94.5)ng/L,各组间血浆8OHdG水平差异有统计学意义(P＜0.001).VaD组患者血浆中8-OHdG水平与对照组比较差异有统计学意义(P＜0.05);而AD组患者血浆中8-OHdG水平与对照组比较差异无统计学意义(P＞0.05);并且VaD组与AD组比较,8-OHdG水平变化的差异也有统计学意义(P＜0.05).各组血浆8-OHdG水平与年龄均呈正相关,分别为:AD组(r=0.661,P＜0.01)、VaD组(r=0.702,P＜0.01)、正常对照组(r=0.719,P＜0.01).而各组血浆8-OHdG水平与教育水平无明显相关性(均P＞0.05).在AD组和VaD组患者中根据其危险因素(性别、吸烟、高血压、糖尿病、高血脂)进行分组,比较各组间血浆8-OHdG水平的差异,结果发现各组间比较差异均无统计学意义(均P＞0.05).校正了性别、年龄、教育水平、吸烟、高血压、高血脂、糖尿病因素的影响后,采用多分类Logistic回归分析结果显示:血浆中高水平8-OHdG在VaD组中比AD组和正常对照组的可能性均高.VaD组血浆8-OHdG水平与MMSE分数呈显著负相关性(r=-0.592,P＜0.01);AD组血浆8-OHdG水平与MMSE分数无相关性(r=-0.122,P＞0.05). 结论 VaD患者存在明显的DNA氧化损伤,其血浆DNA氧化损伤程度显著高于AD患者与健康者;VaD患者的血浆DNA氧化损伤标志物8-OHdG与其认知功能损害程度密切相关.
목적 관찰아이자해묵병(AD)여혈관성치태(VaD)환자혈장중DNA양화손상표지물8간기탈양조감(8-OHdG)수평적변화,탐토DNA양화손상재AD화VaD발병중적작용. 방법 용매련면역흡부시험적방법측정35례AD환자화28례VaD환자이급여지년령상필배적23례건강자혈장중8-OHdG적수평,용간역지역량표(MMSE)평측AD화VaD환자적인지공능. 결과 AD조、VaD조、건강대조조환자혈장중8-OHdG농도분별위(601.8±266.0)ng/L、(1748.0±665.6)ng/L화(352.0±94.5)ng/L,각조간혈장8OHdG수평차이유통계학의의(P＜0.001).VaD조환자혈장중8-OHdG수평여대조조비교차이유통계학의의(P＜0.05);이AD조환자혈장중8-OHdG수평여대조조비교차이무통계학의의(P＞0.05);병차VaD조여AD조비교,8-OHdG수평변화적차이야유통계학의의(P＜0.05).각조혈장8-OHdG수평여년령균정정상관,분별위:AD조(r=0.661,P＜0.01)、VaD조(r=0.702,P＜0.01)、정상대조조(r=0.719,P＜0.01).이각조혈장8-OHdG수평여교육수평무명현상관성(균P＞0.05).재AD조화VaD조환자중근거기위험인소(성별、흡연、고혈압、당뇨병、고혈지)진행분조,비교각조간혈장8-OHdG수평적차이,결과발현각조간비교차이균무통계학의의(균P＞0.05).교정료성별、년령、교육수평、흡연、고혈압、고혈지、당뇨병인소적영향후,채용다분류Logistic회귀분석결과현시:혈장중고수평8-OHdG재VaD조중비AD조화정상대조조적가능성균고.VaD조혈장8-OHdG수평여MMSE분수정현저부상관성(r=-0.592,P＜0.01);AD조혈장8-OHdG수평여MMSE분수무상관성(r=-0.122,P＞0.05). 결론 VaD환자존재명현적DNA양화손상,기혈장DNA양화손상정도현저고우AD환자여건강자;VaD환자적혈장DNA양화손상표지물8-OHdG여기인지공능손해정도밀절상관.
Objective To observe the variation of plasma 8-hydroxy-2'-deoxyguanosine (8-OHdG),a biomarker of oxidative DNA damage,in patients with Alzheimer's disease (AD) versus vascular dementia (VaD),and to investigate the important role and the difference of oxidative DNA damage in the initiation and development of AD and VaD.Methods 35 AD patients,28 VaD patients and 23 age and sex matched normal controls were enrolled in this study.Plasma 8-OHdG was measured by an enzyme-linked immunosorbent assay (ELISA) method.Cognition function in AD and VaD patients was measured by Mini-Mental State Examination (MMSE).Results There were significant differences in plasma 8-OHdG concentrations among AD,VaD and control groups [(601.8±266.0) ng/L,(1748.0±665.6)ng/L,(352.0±94.5)ng/L,respectively,P＜0.001].Plasma 8-OHdG was significantly higher in VaD group than in control group (P＜0.05).While,there was no significant difference in plasma 8OHdG concentration between AD group and control group (P＞0.05).Plasma 8-OHdG was significantly higher in VaD group than in AD group (P＜0.05).Plasma 8-OHdG levels were positively related with age in AD,VaD and control groups (r=0.661,0.702,0.719,respectively,all P％0.01).No correlations were found between plasma 8-OHdG concentration and education level among the three groups (all P＞0.05).AD and VaD patients were subgrouped according to the risk factors including gender,smoking,hypertension,diabetes,hyperlipidemia.There were no significant differences in plasma 8-OHdG level between the subgroups (all P＞ 0.05).After adjusting for sex,age,education level,smoking,hypertension,hyperlipidemia and diabetes,logistic regression analysis showed that the probability of high plasma 8-OHdG level was higher in VaD group than in AD and control groups.Plasma 8-OHdG level had a negative correlation with MMSE score in VaD group (r=-0.592,P＜0.01),while no correlation was found between plasma 8-OHdG level and MMSE score in AD group (r =-0.122,P＞ 0.05).Conclusions There is significant oxidative DNA damage in VaD patients.Plasma oxidative DNA damage is more serious in VaD patients than in AD patients and healthy people.Plasma 8-OHdG level,a oxidative DNA damage biomarker,has a significant correlation with the degree of cognition impairment in VaD patients.