CAJ | 학술논문

　　利用高速逆流色谱(HSCCC)对千层塔提取物废液中含量较高的2个同系物进行初步纯化分离,再利用高效液相色谱(HPLC)对收集到的样品进行同系物分离,得到8 mg化合物A,7 mg化合物 B,纯度均在95%以上。经核磁共振仪(NMR)鉴定2个组分的化学结构,结果证明,千层塔提取物废液所含的主要成分即2个同系物分别为3,4-二羟基肉桂酸和3,4-二甲氧基肉桂酸。结论也同时证实高速逆流色谱与高效液相色谱联用能够有效分离样品所含同系物,且工业提取千层塔有效成分后的废液中仍含有价值物质。
　　이용고속역류색보(HSCCC)대천층탑제취물폐액중함량교고적2개동계물진행초보순화분리,재이용고효액상색보(HPLC)대수집도적양품진행동계물분리,득도8 mg화합물A,7 mg화합물 B,순도균재95%이상。경핵자공진의(NMR)감정2개조분적화학결구,결과증명,천층탑제취물폐액소함적주요성분즉2개동계물분별위3,4-이간기육계산화3,4-이갑양기육계산。결론야동시증실고속역류색보여고효액상색보련용능구유효분리양품소함동계물,차공업제취천층탑유효성분후적폐액중잉함유개치물질。
High-speed counter-current chromatography(HSCCC) is first used preliminarily to purify two homologues with higher content in waste from extracts of Huperzia serrata, and high performance liquid chromatography(HPLC) is used to seperate the two components. 8 mg compound A and 7 mg compound B are obtained, and purity of both compounds reaches more than 95%. Then chemical structure of the two components is identified using the nuclear magnetic resonance apparatus(NMR). The results show that the two components are 3,4-dihydroxy cinnamic acid and 3,4-dimethoxy cinnamic acid. Conclusion can be made that the homologues can be purified by combination of high-speed counter-current chromatography and high performance liquid chromatography, and there still are valuable substances in waste from extracts of Huperzia serrata.