中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2014年
5期
399-403
,共5页
张文%温树信%张涛源%王斌全%高伟%李丽娟
張文%溫樹信%張濤源%王斌全%高偉%李麗娟
장문%온수신%장도원%왕빈전%고위%리려연
乳头状瘤,内翻%PTEN 磷酸水解酶%缺氧诱导因子1,α亚基%恶性变%肿瘤复发,局部
乳頭狀瘤,內翻%PTEN 燐痠水解酶%缺氧誘導因子1,α亞基%噁性變%腫瘤複髮,跼部
유두상류,내번%PTEN 린산수해매%결양유도인자1,α아기%악성변%종류복발,국부
Papilloma,inverted%PTEN phosphohydrolase%Hypoxia-inducible factor 1,alpha subunit%Neoplasm recurrence,local
目的：探讨与张力蛋白和辅助蛋白同源、第10号染色体丢失的磷酸酶基因（ phosphatase and tensin homolog deleted from chromosome ten ， PTEN ）、缺氧诱导因子1α（ hypoxia-inducible factor 1α， HIF-1α）在不同病理分级的鼻腔鼻窦内翻性乳头状瘤（ sinonasal inverted papillomas，SNIP）组织中的表达及意义。方法选取55例SNIP患者的资料，按不同病理分级分为无不典型增生组30例，不典型增生组11例，恶性变组14例。鼻中隔偏曲患者10例作为对照（NM）组。用免疫组化SP法检测SNIP患者乳头状瘤组织和鼻中隔偏曲患者下鼻甲黏膜组织中PTEN和HIF-1α蛋白的表达，分析二者在NM与SNIP之间、不同病理分级的SNIP之间的表达差异，以及PTEN和HIF-1α蛋白的表达与肿瘤复发间的相关性。以SPSS 16.0软件进行统计学分析。结果 PTEN蛋白在NM和SNIP组的阳性率分别为100％、65.5％，差异有统计学意义（U＝147，P＝0.014），HIF-1α蛋白在NM组无表达，在SNIP组的表达率为30.9％，差异有统计学意义（ U＝190，P＝0.045）。在无不典型增生组、不典型增生组、恶性变组中PTEN蛋白的阳性率分别为83.3％、63.6％、28.6％，三组差异有统计学意义（H ＝12.644，P ＝0.002），HIF-1α蛋白在三组的阳性率分别为16.7％、45.5％、50.0％，三组差异有统计学意义（H＝8.292，P＝0.016）。在SNIP组中，PTEN和HIF-1α蛋白的表达呈负相关（r＝-0.503，P＝0.001）。术后随访20～84个月发现，55例患者中22例复发，在术后复发和未复发组中，PTEN蛋白的阳性表达率分别为45.5％、82.8％，差异有统计学意义（χ2＝7.834，P＝0.005）；在术后复发和未复发组中HIF-1α蛋白阳性表达率分别为36.4％、24.1％，差异无统计学意义（χ2＝0.901，P＝0.343）。结论随着SNIP恶性程度的增加，PTEN蛋白表达减少，HIF-1α蛋白表达增加；PTEN和HIF-1α可能共同参与了SNIP的进展及恶性变过程；PTEN是影响SNIP术后复发的重要因素。
目的：探討與張力蛋白和輔助蛋白同源、第10號染色體丟失的燐痠酶基因（ phosphatase and tensin homolog deleted from chromosome ten ， PTEN ）、缺氧誘導因子1α（ hypoxia-inducible factor 1α， HIF-1α）在不同病理分級的鼻腔鼻竇內翻性乳頭狀瘤（ sinonasal inverted papillomas，SNIP）組織中的錶達及意義。方法選取55例SNIP患者的資料，按不同病理分級分為無不典型增生組30例，不典型增生組11例，噁性變組14例。鼻中隔偏麯患者10例作為對照（NM）組。用免疫組化SP法檢測SNIP患者乳頭狀瘤組織和鼻中隔偏麯患者下鼻甲黏膜組織中PTEN和HIF-1α蛋白的錶達，分析二者在NM與SNIP之間、不同病理分級的SNIP之間的錶達差異，以及PTEN和HIF-1α蛋白的錶達與腫瘤複髮間的相關性。以SPSS 16.0軟件進行統計學分析。結果 PTEN蛋白在NM和SNIP組的暘性率分彆為100％、65.5％，差異有統計學意義（U＝147，P＝0.014），HIF-1α蛋白在NM組無錶達，在SNIP組的錶達率為30.9％，差異有統計學意義（ U＝190，P＝0.045）。在無不典型增生組、不典型增生組、噁性變組中PTEN蛋白的暘性率分彆為83.3％、63.6％、28.6％，三組差異有統計學意義（H ＝12.644，P ＝0.002），HIF-1α蛋白在三組的暘性率分彆為16.7％、45.5％、50.0％，三組差異有統計學意義（H＝8.292，P＝0.016）。在SNIP組中，PTEN和HIF-1α蛋白的錶達呈負相關（r＝-0.503，P＝0.001）。術後隨訪20～84箇月髮現，55例患者中22例複髮，在術後複髮和未複髮組中，PTEN蛋白的暘性錶達率分彆為45.5％、82.8％，差異有統計學意義（χ2＝7.834，P＝0.005）；在術後複髮和未複髮組中HIF-1α蛋白暘性錶達率分彆為36.4％、24.1％，差異無統計學意義（χ2＝0.901，P＝0.343）。結論隨著SNIP噁性程度的增加，PTEN蛋白錶達減少，HIF-1α蛋白錶達增加；PTEN和HIF-1α可能共同參與瞭SNIP的進展及噁性變過程；PTEN是影響SNIP術後複髮的重要因素。
목적：탐토여장력단백화보조단백동원、제10호염색체주실적린산매기인（ phosphatase and tensin homolog deleted from chromosome ten ， PTEN ）、결양유도인자1α（ hypoxia-inducible factor 1α， HIF-1α）재불동병리분급적비강비두내번성유두상류（ sinonasal inverted papillomas，SNIP）조직중적표체급의의。방법선취55례SNIP환자적자료，안불동병리분급분위무불전형증생조30례，불전형증생조11례，악성변조14례。비중격편곡환자10례작위대조（NM）조。용면역조화SP법검측SNIP환자유두상류조직화비중격편곡환자하비갑점막조직중PTEN화HIF-1α단백적표체，분석이자재NM여SNIP지간、불동병리분급적SNIP지간적표체차이，이급PTEN화HIF-1α단백적표체여종류복발간적상관성。이SPSS 16.0연건진행통계학분석。결과 PTEN단백재NM화SNIP조적양성솔분별위100％、65.5％，차이유통계학의의（U＝147，P＝0.014），HIF-1α단백재NM조무표체，재SNIP조적표체솔위30.9％，차이유통계학의의（ U＝190，P＝0.045）。재무불전형증생조、불전형증생조、악성변조중PTEN단백적양성솔분별위83.3％、63.6％、28.6％，삼조차이유통계학의의（H ＝12.644，P ＝0.002），HIF-1α단백재삼조적양성솔분별위16.7％、45.5％、50.0％，삼조차이유통계학의의（H＝8.292，P＝0.016）。재SNIP조중，PTEN화HIF-1α단백적표체정부상관（r＝-0.503，P＝0.001）。술후수방20～84개월발현，55례환자중22례복발，재술후복발화미복발조중，PTEN단백적양성표체솔분별위45.5％、82.8％，차이유통계학의의（χ2＝7.834，P＝0.005）；재술후복발화미복발조중HIF-1α단백양성표체솔분별위36.4％、24.1％，차이무통계학의의（χ2＝0.901，P＝0.343）。결론수착SNIP악성정도적증가，PTEN단백표체감소，HIF-1α단백표체증가；PTEN화HIF-1α가능공동삼여료SNIP적진전급악성변과정；PTEN시영향SNIP술후복발적중요인소。
Objective To investigate the expression and clinical significance of phosphatase and tensin homology deleted on ehromosome ten ( PTEN ) and hypoxia-inducible factor 1 alpha ( HIF-1α) in sinonasal inverted papilloma (SNIP) of different pathological grades.Methods Fifty-five paraffin samples from patients with SNIP and a control group of 10 paraffin samples of patients with nomal nasal cavity mucosa (NM) who underwent inferior turbinectomy were consisted in this study.Among the 55 cases of SNIP, 30 cases were without dysplasia subtypes , 11 cases were with dysplasia subtypes , and 14 cases with canceration to squamous cell carcinoma ( SCC ) subtypes.PTEN and HIF-1αexpression in SNIP was detected by immunohistochemistry.The differences between NM and SNIP , and among the three subtypes were analyzed , and the relationship between PTEN , HIF-1αexpression and SNIP recurrence and the correlation between PTEN expression and HIF-1αexpression were also analyzed.SPSS 16.0 software was used to analyze the data.Results The positive expression rate of PTEN in NM and SNIP was 100% and 65.5%, the difference was significant ( U =147, P =0.014), while HIF-1αwas 0 and 30.9%, the difference was significant (U=190, P=0.045).The positive expression rate of PTEN in SNIP without dysplasia, SNIP with dysplasia and NSCC was 83.3%, 63.6%, 28.6%, respectively, the difference was significant(H =12.644, P =0.002); while HIF-1αwas 16.7%, 45.5%, 50.0%, respectively, the difference was significant(H=8.292, P=0.016).A total of 22 SNIP patients recurred.PTEN had lower expression in recurrent SNIP ( 45.5%) than that in non-recurrent SNIP ( 82.8%) , and the difference was significant(χ2 =7.834, P =0.005).However, the expression of HIF-1αhad no significant difference between recurrent SNIP and the SNIP which had no recurrence (χ2 =0.901, P=0.343).The expression of PTEN protein was negatively correlated with that of HIF-1αprotein (r=-0.503, P=0.001).Conclusions PTEN expression decreased graduately with the severity of malignancy of SNIP , but HIF-1αincreased.The expression of HIF-1αwas induced by hypoxia , which may negatively effect the expression of PTEN , and both HIF-1αand PTEN may play critical roles in the progress of SNIP.PTEN is one of the factors responsible for the postoperative recurrence of SNIP.
