CAJ | 학술논문

　　以玉米植株为材料，对提取、纯化和利用高效液相色谱法分析测定玉米植株中内源激素生长素(IAA)、脱落酸(ABA)、赤霉素(GA3)和玉米素(ZT)的含量的方法进行改进。针对玉米植株的糖分、叶绿素含量高等特点，对以往的HPLC分析植物内源激素的植物材料前处理方法进行改进。试材用液氮研磨以80%预冷的甲醇浸提，浸提液利用旋转蒸发仪浓缩、石油醚去除色素、乙酸乙酯提取激素、PVP 去除酚类杂质，最后用0.45 um微孔滤膜抽滤，紫外检测器在254 nm波长下检测吸收波长，以甲醇和水位流动相，利用高效液相色谱法检测内源激素含量，回收率分别为IAA 93.79%±7.43%，ABA 95.79%±6.14%，GA388.63%±6.31%，ZT 89.69%±7.34%。
　　이옥미식주위재료，대제취、순화화이용고효액상색보법분석측정옥미식주중내원격소생장소(IAA)、탈락산(ABA)、적매소(GA3)화옥미소(ZT)적함량적방법진행개진。침대옥미식주적당분、협록소함량고등특점，대이왕적HPLC분석식물내원격소적식물재료전처리방법진행개진。시재용액담연마이80%예랭적갑순침제，침제액이용선전증발의농축、석유미거제색소、을산을지제취격소、PVP 거제분류잡질，최후용0.45 um미공려막추려，자외검측기재254 nm파장하검측흡수파장，이갑순화수위류동상，이용고효액상색보법검측내원격소함량，회수솔분별위IAA 93.79%±7.43%，ABA 95.79%±6.14%，GA388.63%±6.31%，ZT 89.69%±7.34%。
This experiment improved the method of extraction, purification and analysis by high-performance liquid chromatography(HPLC) on endogenous hormones auxin(IAA), abscisic acid(ABA) and gibberellic acid(GA3) and zeatin(ZT) content in maize plant tissues. To deal with the existence of large amount of sugar and chlorophyll. The test materials were grinded in liquid nitrogen and lixiviated in 80%methanol, then pigment was eliminated in petroleum ether, phytohormones were extracted in ethyl acetate, phenolic was compounded in polyving pyrrolidone(PVP) and quantified by 0.45 um microporous membrane filter. Detection was made by UV detector at 254 nm. Reversed HPLC with methanol-water at a flow rate of 1.0 mL/min. The frequency of reclamation was IAA 93.79%±7.43%, ABA 95.79%±6.14%, GA3 88.63%±6.31%, ZT 89.69%±7.34%.