CAJ | 학술논문

　　土壤盐碱化是影响玉米产量的重要非生物因素之一。用200 mmol/L NaCl溶液对耐盐玉米自交系E28进行盐处理，提取叶片可溶性蛋白质进行双向电泳，并用ImageMasterTM 2D 6.00软件分析，探究玉米的耐盐机理。结果表明，盐胁迫处理后共有10个有明显差异的蛋白点，其中4个表达上调，6个表达下调。质谱分析和数据库搜索鉴定出应激反应蛋白和类囊体腔19 KDa蛋白，可能是盐胁迫后植物营养生长及光合作用受到影响的结果。
　　토양염감화시영향옥미산량적중요비생물인소지일。용200 mmol/L NaCl용액대내염옥미자교계E28진행염처리，제취협편가용성단백질진행쌍향전영，병용ImageMasterTM 2D 6.00연건분석，탐구옥미적내염궤리。결과표명，염협박처리후공유10개유명현차이적단백점，기중4개표체상조，6개표체하조。질보분석화수거고수색감정출응격반응단백화류낭체강19 KDa단백，가능시염협박후식물영양생장급광합작용수도영향적결과。
Soil salinization is one of the most important abiotic constraints in maize yield. Seedlings of salini-ty-tolerant maize inbred line E28 were treated with 200 mmol/L NaCl solution and the soluble proteins of leaves were extracted by TCA/acetone method for two-dimensional gel electrophoresis. Software ImageMasterTM 2D 6.00 was used to analyze the results. There were 4 up-expressed spots and 6 down-expressed spots in total 10 significantly different proteins. MALDI-TOF-MS analysis indicated that there were stress responsive protein and thylakoid lumenal 19 kDa protein, which were considered as the results of changes in maize growth and photosynthesis.