CAJ | 학술논문

　　采用生物信息学的方法，在玉米全基因组水平鉴定并分析玉米LIM蛋白基因，鉴定出11个ZmLIM基因，分别分布于玉米的8条染色体上。结构域分析表明，多数LIM蛋白包括2个典型的LIM结构域。系统发育分析表明，LIM家族可以分为3个亚家族。假定调控元件分析发现，所有LIM基因启动子都具有大量花粉特异表达元件，相对来说其他组织器官特异表达，激素和逆境调控元件数量很少。EST基因表达数据分析表明，该家族成员具有明显的时空表达特性。
　　채용생물신식학적방법，재옥미전기인조수평감정병분석옥미LIM단백기인，감정출11개ZmLIM기인，분별분포우옥미적8조염색체상。결구역분석표명，다수LIM단백포괄2개전형적LIM결구역。계통발육분석표명，LIM가족가이분위3개아가족。가정조공원건분석발현，소유LIM기인계동자도구유대량화분특이표체원건，상대래설기타조직기관특이표체，격소화역경조공원건수량흔소。EST기인표체수거분석표명，해가족성원구유명현적시공표체특성。
By bioinformatic analysis, maize LIM genes were isolated and analyzed in those functions in whole-genome scale. A total of 11 maize LIM genes(ZmLIM1 to ZmLIM11) were identified. ZmLIM genes were dis-tributed in all the maize chromosomes except chromosome 7 and 8. Alignment and motif analysis results revealed ma-jor maize LIM proteins share two typical conserved domains. Phylogenetic analysis indicated LIM family could be di-vided into three subfamilies. Putative cis-acting regulatory elements involved in specific tissue or organ, phytohor-mones and stress, the tissue-specific expression elements, especial pollen-specific element, were observed in the pro-moters of all ZmLIM genes; other elements distribution presented fragmentarily. Expression data of ESTs suggested maize LIM genes had temporal and spatial expression pattern.