CAJ | 학술논문

　　采用染色体步移技术(Genome Walking)分离转Bt-pta-bar基因玉米JL937的插入位点侧翼序列，获得了615 bp的5’端边界序列。据此序列设计特异性引物，建立了JL937玉米的特异性定性PCR检测方法，可特异性地从JL937玉米中扩增出221 bp的产物。此方法具有特异性强、稳定性好的特点，适用于JL937玉米的定性检测。
　　채용염색체보이기술(Genome Walking)분리전Bt-pta-bar기인옥미JL937적삽입위점측익서렬，획득료615 bp적5’단변계서렬。거차서렬설계특이성인물，건립료JL937옥미적특이성정성PCR검측방법，가특이성지종JL937옥미중확증출221 bp적산물。차방법구유특이성강、은정성호적특점，괄용우JL937옥미적정성검측。
The 5’-transgene integration sequence between the host plant DNA and the integrated gene construct of the GM maize JL937 was revealed by means of Genome Walking technology, and a 615 bp flanking sequence was arrived. According to this sequence, the specific PCR primers were designed, and the conventional qualitative PCR method was successfully developed. The validation results indicated that this established method was reliable, specific, and suitable for the qualitative detection of JL937 event.