齐鲁工业大学学报
齊魯工業大學學報
제로공업대학학보
Journal of Shandong Institute of Light Industry (Natural Science Edition)
2013年
3期
20-24
,共5页
酒花多酚%丙酮%乙醇%水杨酸法%羟自由基
酒花多酚%丙酮%乙醇%水楊痠法%羥自由基
주화다분%병동%을순%수양산법%간자유기
hop polyphenols%acetone%ethanol%salicylic acid assay%hydroxyl radical
分别用70％丙酮水溶液和50％乙醇水溶液对酒花中的多酚类化合物进行提取，并采用福林酚法测定提取液中总多酚的含量，发现70％丙酮对酒花多酚提取量（40．15 mg／g酒花）比50％乙醇对酒花多酚提取量（31．18 mg／g酒花）高出11％。选用水杨酸法测定了以上两种酒花多酚提取物对羟自由基的清除率，并以抗坏血酸和茶多酚做阳性对照。结果表明，酒花多酚对羟自由基具有较强的清除作用，其清除率在0～100μg／mL的多酚浓度范围内随浓度的增加而增大，呈现明显的线性关系，各试样对羟自由基清除力大小顺序依次为：50％乙醇酒花多酚（ IC50值为11．8μg／mL）＞抗坏血酸（IC50值为33．5μg／mL）＞茶多酚（IC50值为42．0μg／mL）＞70％丙酮酒花多酚（IC50值为58．4μg／mL）。当试样浓度达到0．2 mg／mL时，50％乙醇酒花多酚对羟自由基清除率为99．7％，与Vc和茶多酚没有显著差异，但显著高于70％丙酮酒花多酚（清除率为93．8％）。结论：极性大的酒花多酚组分其抗氧化活性高于极性小的酒花多酚组分。
分彆用70％丙酮水溶液和50％乙醇水溶液對酒花中的多酚類化閤物進行提取，併採用福林酚法測定提取液中總多酚的含量，髮現70％丙酮對酒花多酚提取量（40．15 mg／g酒花）比50％乙醇對酒花多酚提取量（31．18 mg／g酒花）高齣11％。選用水楊痠法測定瞭以上兩種酒花多酚提取物對羥自由基的清除率，併以抗壞血痠和茶多酚做暘性對照。結果錶明，酒花多酚對羥自由基具有較彊的清除作用，其清除率在0～100μg／mL的多酚濃度範圍內隨濃度的增加而增大，呈現明顯的線性關繫，各試樣對羥自由基清除力大小順序依次為：50％乙醇酒花多酚（ IC50值為11．8μg／mL）＞抗壞血痠（IC50值為33．5μg／mL）＞茶多酚（IC50值為42．0μg／mL）＞70％丙酮酒花多酚（IC50值為58．4μg／mL）。噹試樣濃度達到0．2 mg／mL時，50％乙醇酒花多酚對羥自由基清除率為99．7％，與Vc和茶多酚沒有顯著差異，但顯著高于70％丙酮酒花多酚（清除率為93．8％）。結論：極性大的酒花多酚組分其抗氧化活性高于極性小的酒花多酚組分。
분별용70％병동수용액화50％을순수용액대주화중적다분류화합물진행제취，병채용복림분법측정제취액중총다분적함량，발현70％병동대주화다분제취량（40．15 mg／g주화）비50％을순대주화다분제취량（31．18 mg／g주화）고출11％。선용수양산법측정료이상량충주화다분제취물대간자유기적청제솔，병이항배혈산화다다분주양성대조。결과표명，주화다분대간자유기구유교강적청제작용，기청제솔재0～100μg／mL적다분농도범위내수농도적증가이증대，정현명현적선성관계，각시양대간자유기청제력대소순서의차위：50％을순주화다분（ IC50치위11．8μg／mL）＞항배혈산（IC50치위33．5μg／mL）＞다다분（IC50치위42．0μg／mL）＞70％병동주화다분（IC50치위58．4μg／mL）。당시양농도체도0．2 mg／mL시，50％을순주화다분대간자유기청제솔위99．7％，여Vc화다다분몰유현저차이，단현저고우70％병동주화다분（청제솔위93．8％）。결론：겁성대적주화다분조분기항양화활성고우겁성소적주화다분조분。
Polyphenols in hops were extracted by 70%acetone and 50%ethanol,respectively.The content of total polyphenols in extract was determined by Folin-Cioealteu assay .It was found that the extract quality of hop polyphenols with 50%ethanol was 40.15 mg/g hop,witch was over 11%,than that with 70%acetone was 31.18 mg/g hop.In this study,salicylic acid assay was performed to determining hydroxyl radical scavenging activity of the extract ,ascorbic acid and tea polyphenols as the positive control .The analyzed results indicated that the extract of hop polyphenols has strong hydroxyl radical scavenging activity .And the concentration rangs from 0 to 100μg/mL,the scavenging activity by hop polyphenols was increasing showing a linear relationship . The effect of samples on hydroxyl radical scavenging activity was followed by:50%ethanol extract ( IC50 was 11.8μg/mL) >Vc ( IC50 was 33.5 μg/mL) >tea polyphenols ( IC50 was 42.0 μg/mL) >70%acetone (IC50 was 58.4 μg/mL).When the concentration of sample up to 0.2 mg/mL,the scavenging rate of hop extract with 50%ethanol was 99.7%,there is no significant difference compared with Vc and tea polyphenols , but significantly higher than those of hop extract with 50%acetone(scavenging rate is 93.8%).It is suggested that hop polyphenols composition extracted by ethanol with strong polarity has better antioxidant activity than those extracted by acetone with little polarity .
