齐鲁工业大学学报
齊魯工業大學學報
제로공업대학학보
Journal of Shandong Institute of Light Industry (Natural Science Edition)
2013年
3期
46-50
,共5页
于向荣%汤小宁%于清琴%孔健%钱洋
于嚮榮%湯小寧%于清琴%孔健%錢洋
우향영%탕소저%우청금%공건%전양
乳酸菌%发酵液%抑菌%炭疽病%白腐病
乳痠菌%髮酵液%抑菌%炭疽病%白腐病
유산균%발효액%억균%탄저병%백부병
lactic acid bacteria%fermentation broth%bacteriostat%grape anthracnose%grape white rot
本实验从保存的乳酸菌菌株中筛选出了抑霉菌效果好、抑菌谱较广的三株乳酸菌(2025b、2026b1、6-1-1),研究了三株乳酸菌菌株发酵液的详细性质,包括生长曲线的绘制,发酵过程中pH值的变化情况,发酵液对蛋白酶的稳定性,发酵液对高温的稳定性,测试了发酵液随着储存时间的延长形成的抑菌能力变化情况。利用筛选出的三株乳酸菌(2025b、2026b1、6-1-1)发酵产生的无菌发酵液进行面包防腐实验和葡萄炭疽病和白腐病病菌抑菌试验,发现乳酸菌发酵液对面包保鲜期限的延长效果明显,对葡萄炭疽病和白腐病的病菌抑制效果明显。
本實驗從保存的乳痠菌菌株中篩選齣瞭抑黴菌效果好、抑菌譜較廣的三株乳痠菌(2025b、2026b1、6-1-1),研究瞭三株乳痠菌菌株髮酵液的詳細性質,包括生長麯線的繪製,髮酵過程中pH值的變化情況,髮酵液對蛋白酶的穩定性,髮酵液對高溫的穩定性,測試瞭髮酵液隨著儲存時間的延長形成的抑菌能力變化情況。利用篩選齣的三株乳痠菌(2025b、2026b1、6-1-1)髮酵產生的無菌髮酵液進行麵包防腐實驗和葡萄炭疽病和白腐病病菌抑菌試驗,髮現乳痠菌髮酵液對麵包保鮮期限的延長效果明顯,對葡萄炭疽病和白腐病的病菌抑製效果明顯。
본실험종보존적유산균균주중사선출료억매균효과호、억균보교엄적삼주유산균(2025b、2026b1、6-1-1),연구료삼주유산균균주발효액적상세성질,포괄생장곡선적회제,발효과정중pH치적변화정황,발효액대단백매적은정성,발효액대고온적은정성,측시료발효액수착저존시간적연장형성적억균능력변화정황。이용사선출적삼주유산균(2025b、2026b1、6-1-1)발효산생적무균발효액진행면포방부실험화포도탄저병화백부병병균억균시험,발현유산균발효액대면포보선기한적연장효과명현,대포도탄저병화백부병적병균억제효과명현。
In the experiment,three strains of lactic acid bacteria (2025b,2026b1,6-1-1) were selected in the all Lactic acid bacteria strains saved , which had an effective bacteriostasis and broad antifungal spectrum.The qualities of the fermentation broth of the three Lactic acid bacteria ,were studied including the growth curve of Lactobacillus , the change of pH value during Ferment , the stability of fermentation broth towards protease ,and the stability of fermentation broth towards high temperature .Besides,how the antibacterial activities of fermentation broth change was tested when the storage time was prolonged .The sterile formentalion mediam was made by the tree strains of lactic acid bacteria (2025b、2026b、6-1-1) to conduct the experiments about bread preservation and grape anthracnose inhibition and grape white rot of bacteriostasis ,it is found that the fermentation broth benefits both bread preservation and antibacterial effect of grape anthracnose and grape white rot .