CAJ | 학술논문

　　以郑单958和丰单3号的3展叶期幼苗为材料，在人工气候室进行盆栽试验，研究4℃低温胁迫至幼苗死亡过程中叶片氮素代谢关键酶活的变化规律及聚糠萘水剂(PKN)的调控效应。结果表明，低温胁迫打破了氮素代谢平衡，致使氮素同化相关酶硝酸还原酶活性(NRA)、谷氨酰胺合成酶活性(GSA)和谷氨酸-草酰乙酸转移酶活性(GOTA)于胁迫初期升高，之后迅速下降，且GSA和GOTA降低比NRA提前2 d。随低温胁迫时间的延长，玉米幼苗受害加重，蛋白酶、肽酶活性增加，叶片可溶性蛋白与游离氨基酸含量升高。PKN处理提高了NRA、GSA、GOTA，降低了蛋白酶、肽酶活性，维持氮素代谢稳定，促进单株干物重增加，提高了玉米幼苗抗冷性。
　　이정단958화봉단3호적3전협기유묘위재료，재인공기후실진행분재시험，연구4℃저온협박지유묘사망과정중협편담소대사관건매활적변화규률급취강내수제(PKN)적조공효응。결과표명，저온협박타파료담소대사평형，치사담소동화상관매초산환원매활성(NRA)、곡안선알합성매활성(GSA)화곡안산-초선을산전이매활성(GOTA)우협박초기승고，지후신속하강，차GSA화GOTA강저비NRA제전2 d。수저온협박시간적연장，옥미유묘수해가중，단백매、태매활성증가，협편가용성단백여유리안기산함량승고。PKN처리제고료NRA、GSA、GOTA，강저료단백매、태매활성，유지담소대사은정，촉진단주간물중증가，제고료옥미유묘항랭성。
A pot experiment of maize seedlings(material:Zhengdan958 and Fengdan3) was conducted in artificial climate chamber to investigate the effects of low temperature stress and the chemical regulator PKN with various nitro-gen metabolism key enzymes activities. The results showed that the low temperature stress broken nitrogen metabolism balance and made NRA, GSA, GOTA significantly increasing in the beginning 2-3 days of the low temperature pres-sure, and then descended quickly. GSA and GOTA were faster than NRA 2 days. Low temperature stress increased protease and peptidase activity, promote protein decomposed. And soluble protein and free amino acid content in-creased. PKN pretreatment improved NRA, GSA, GOTA, maintained the stability of nitrogen metabolism and reduce the protease, peptidase activity, promoted the dry weight increased, improved the maize seedling cold resistance.