玉米科学
玉米科學
옥미과학
JOURNAL OF MAIZE SCIENCES
2013年
4期
28-32
,共5页
宋伟%王凤格%田红丽%易红梅%王璐%赵久然
宋偉%王鳳格%田紅麗%易紅梅%王璐%趙久然
송위%왕봉격%전홍려%역홍매%왕로%조구연
玉米%自交系%SNP%品种鉴定
玉米%自交繫%SNP%品種鑒定
옥미%자교계%SNP%품충감정
Maize%Inbred line%SNP%Variety identification
根据多态性水平、染色体位置等信息从公共数据库上筛选了48个玉米核心SNP位点。利用48重-SNPLex分型系统对105份玉米自交系进行SNP基因分型分析,探索SNP标记在玉米品种鉴定中的应用前景。结果表明,48个SNP位点中有42个位点峰型正常;PIC值在0.019~0.375之间,平均为0.242;任何两份自交系间的遗传距离均在0.015以上,即42个SNP位点的基因分型数据信息可以将105份自交系材料区分开。
根據多態性水平、染色體位置等信息從公共數據庫上篩選瞭48箇玉米覈心SNP位點。利用48重-SNPLex分型繫統對105份玉米自交繫進行SNP基因分型分析,探索SNP標記在玉米品種鑒定中的應用前景。結果錶明,48箇SNP位點中有42箇位點峰型正常;PIC值在0.019~0.375之間,平均為0.242;任何兩份自交繫間的遺傳距離均在0.015以上,即42箇SNP位點的基因分型數據信息可以將105份自交繫材料區分開。
근거다태성수평、염색체위치등신식종공공수거고상사선료48개옥미핵심SNP위점。이용48중-SNPLex분형계통대105빈옥미자교계진행SNP기인분형분석,탐색SNP표기재옥미품충감정중적응용전경。결과표명,48개SNP위점중유42개위점봉형정상;PIC치재0.019~0.375지간,평균위0.242;임하량빈자교계간적유전거리균재0.015이상,즉42개SNP위점적기인분형수거신식가이장105빈자교계재료구분개。
Based on the level of polymorphism and chromosome location, forty-eight maize core SNP loci were screened from public databases. To explore the application prospect of SNP marker in maize variety identification, SNP genotypic analysis of 105 maize inbred lines was finished on 48 X-SNPLexTM genotypic system. The results showed that 42 of 48 SNP loci have normal peak. The polymorphism information content(PIC) of past loci varied from 0.019 to 0.375 with an average of 0.242. The result showed that genetic distance between any two inbred lines was more than 0.015. In other words, while more SNP loci may be needed for variety discrimination in populations with high consanguinity level, such as essential derivation varieties, forty-two SNP loci panel presents enough power for i-dentity control of the 105 major inbred lines.