中国农学通报
中國農學通報
중국농학통보
CHINESE AGRICULTURAL SCIENCE BULLETIN
2013年
13期
153-157
,共5页
吴昀%马怡迪%张琳%夏宜平
吳昀%馬怡迪%張琳%夏宜平
오윤%마이적%장림%하의평
药百合%激素处理%种子剥皮处理%种子萌发%染色体倍性
藥百閤%激素處理%種子剝皮處理%種子萌髮%染色體倍性
약백합%격소처리%충자박피처리%충자맹발%염색체배성
Lilium speciosum var. gloriosoides%hormone treatment%seeds germination%chromosome ploidy
旨在研究药百合种子萌发的适宜激素配比。以浙江省广布种药百合为材料，采用不同激素浓度的培养基进行种子诱导及萌发实验，以不添加任何激素的MS培养基为对照，对药百合种子萌发率、诱导率、诱导芽数及生长势等进行研究，同时，对药百合种子诱导所形成试管苗进行染色体制片常规技术分析。结果表明，添加不同激素浓度培养基能显著促进药百合种子萌发，最佳培养基为MS+6-BA1.0 mg/L+NAA 0.1 mg/L，萌发率为83.3%，诱导率为88.9%，平均诱导不定芽数达2.5个；未经剥皮处理的种子在30天时仍无任何萌动；将诱导后的种子转至MS基本培养基中，仍表现出与转接前一致的生长状态，说明百合种皮是制约百合种子萌发的关键因素，进行剥皮处理或其他物理措施可有效提高其萌发率；种子萌发试管苗染色体倍性为2n=2x=24，未发生遗传变异。
旨在研究藥百閤種子萌髮的適宜激素配比。以浙江省廣佈種藥百閤為材料，採用不同激素濃度的培養基進行種子誘導及萌髮實驗，以不添加任何激素的MS培養基為對照，對藥百閤種子萌髮率、誘導率、誘導芽數及生長勢等進行研究，同時，對藥百閤種子誘導所形成試管苗進行染色體製片常規技術分析。結果錶明，添加不同激素濃度培養基能顯著促進藥百閤種子萌髮，最佳培養基為MS+6-BA1.0 mg/L+NAA 0.1 mg/L，萌髮率為83.3%，誘導率為88.9%，平均誘導不定芽數達2.5箇；未經剝皮處理的種子在30天時仍無任何萌動；將誘導後的種子轉至MS基本培養基中，仍錶現齣與轉接前一緻的生長狀態，說明百閤種皮是製約百閤種子萌髮的關鍵因素，進行剝皮處理或其他物理措施可有效提高其萌髮率；種子萌髮試管苗染色體倍性為2n=2x=24，未髮生遺傳變異。
지재연구약백합충자맹발적괄의격소배비。이절강성엄포충약백합위재료，채용불동격소농도적배양기진행충자유도급맹발실험，이불첨가임하격소적MS배양기위대조，대약백합충자맹발솔、유도솔、유도아수급생장세등진행연구，동시，대약백합충자유도소형성시관묘진행염색체제편상규기술분석。결과표명，첨가불동격소농도배양기능현저촉진약백합충자맹발，최가배양기위MS+6-BA1.0 mg/L+NAA 0.1 mg/L，맹발솔위83.3%，유도솔위88.9%，평균유도불정아수체2.5개；미경박피처리적충자재30천시잉무임하맹동；장유도후적충자전지MS기본배양기중，잉표현출여전접전일치적생장상태，설명백합충피시제약백합충자맹발적관건인소，진행박피처리혹기타물리조시가유효제고기맹발솔；충자맹발시관묘염색체배성위2n=2x=24，미발생유전변이。
The aim was to study the proper ratio of hormones for seed germination. Lilium speciosum var. gloriosoides widely distributed in Zhejiang Province, was used as matericals. The seeds of induction and germination medium adding different concentration of hormones were studied, and the MS basal medium without any hormones was used as control. The germination rate, shoot induction rate, number of shoots inducted were measured, and the growth of plantlets were observed during the experiment. Meanwhile, the seeds induced form plantlets were analyzed using the conventional chromosome preparation in this paper. The results showed that medium adding hormones could enhance the germination rate significantly, the optimum medium was MS media supplemented with 6-BA 1.0 mg/L and NAA 0.1 mg/L, the germination percentage, induction rate and average indefinite buds were 88.3%, 88.9% and 2.5, respectively. Seeds without peeling treatment did not germinate after inoculating 30 days at all. The induced seedlings were transferred to MS medium, the growth condition sustained as before, which illustrated testa was the key factor limiting seed germination and hormone treatment or other physical measures can effectively improve the germination rate. The ploidy levels of seeds induction plantlets were diploid (2n=2x=24), and no genetic variation was found.
