上海医药
上海醫藥
상해의약
SHANGHAI MEDICAL & PHARMACEUTICAL JOURNAL
2013年
13期
50-52,61
,共4页
尹大全%袁晖%黄君勤
尹大全%袁暉%黃君勤
윤대전%원휘%황군근
硫酸黏菌素%多黏菌素B2%分离纯化
硫痠黏菌素%多黏菌素B2%分離純化
류산점균소%다점균소B2%분리순화
colistin sulfate%polymyxin B2%isolation and purification
目的：研究本公司硫酸黏菌素有效组分与欧洲药典标准品的区别。方法：采用高效液相色谱法对样品进行对比分析，目标组分经制备液相分离纯化后通过核磁共振和质谱进行结构解析。结果：分离得到的与欧洲药典标准品不一致的组分为多黏菌素B2。结论：发酵菌种不一致可能会导致有效组分有一定的差别，应该引起生产厂家的关注。
目的：研究本公司硫痠黏菌素有效組分與歐洲藥典標準品的區彆。方法：採用高效液相色譜法對樣品進行對比分析，目標組分經製備液相分離純化後通過覈磁共振和質譜進行結構解析。結果：分離得到的與歐洲藥典標準品不一緻的組分為多黏菌素B2。結論：髮酵菌種不一緻可能會導緻有效組分有一定的差彆，應該引起生產廠傢的關註。
목적：연구본공사류산점균소유효조분여구주약전표준품적구별。방법：채용고효액상색보법대양품진행대비분석，목표조분경제비액상분리순화후통과핵자공진화질보진행결구해석。결과：분리득도적여구주약전표준품불일치적조분위다점균소B2。결론：발효균충불일치가능회도치유효조분유일정적차별，응해인기생산엄가적관주。
Objective:To study the difference of active ingredients in colistin sulfate produced by Xinyapharm and European Pharmacopoeia Reference Standard. Methods:The desired components were isolated and purified by preparative HPLC and the structure of one of the components was elucidated based on the analysis of NMR and MS data. Results:The component which does not exist in colistin sulfate from European Pharmacopoeia Reference Standard is polymyxin B2. Conclusion:The difference of active ingredient could be caused by different microbial strain, to which close attention should be paid by manufactures.
