CAJ | 학술논문

目的：检测舌癌组织中，血管内皮生长因子（VEGF）的表达情况及微血管密度，以探讨VEGF水平与舌癌浸润转移与复发的关系。方法收集舌癌病人44例，均经组织病理学诊断为鳞状细胞癌，并确定其肿瘤类型、分化程度及有无淋巴结转移。良性对照组为13例舌乳头状瘤患者。采用S-P免疫组化法，对44例舌癌及13例舌乳头状瘤进行VEGF单克隆抗体和CD34单克隆抗体染色，在镜下进行VEGF分级及微血管计数。结果①44例舌癌VEGF染色结果：20例（-），16例（＋），8例（++），微血管密度均值为39．18/200×视野（SD＝3．62），两者呈正相关。②在颈淋巴结转移组与未转移组间，VEGF表达、MVD、肿瘤大小及临床分期均有显著差异，但在统计学上，VEGF与MVD的意义较大。③在复发组与未复发组间，VEGF与MVD存在显著差异。结论VEGF及MVD与舌癌浸润转移及复发间存在密切的关系。
목적：검측설암조직중，혈관내피생장인자（VEGF）적표체정황급미혈관밀도，이탐토VEGF수평여설암침윤전이여복발적관계。방법수집설암병인44례，균경조직병이학진단위린상세포암，병학정기종류류형、분화정도급유무림파결전이。량성대조조위13례설유두상류환자。채용S-P면역조화법，대44례설암급13례설유두상류진행VEGF단극륭항체화CD34단극륭항체염색，재경하진행VEGF분급급미혈관계수。결과①44례설암VEGF염색결과：20례（-），16례（＋），8례（++），미혈관밀도균치위39．18/200×시야（SD＝3．62），량자정정상관。②재경림파결전이조여미전이조간，VEGF표체、MVD、종류대소급림상분기균유현저차이，단재통계학상，VEGF여MVD적의의교대。③재복발조여미복발조간，VEGF여MVD존재현저차이。결론VEGF급MVD여설암침윤전이급복발간존재밀절적관계。
Objective To detect expression of vascular endothelial growth factor (VEGF) and microvessel density(MVD) in tongue squamous cell carcinoma ,in order to explore the relationship between VEGF level and the infiltrative metastasis and recurrence .Meth-ods Forty-four tongue cancer patients were enrolled,which were squamous cell carcinoma,and determine the type of tumor,differentiation grade and lymph node metastasis were showed .The positive control group was 13 tongue papilloma patients .Immunohistochemical S-P was used to observe the staining results .Results ①The results of VEGF staining in 44 patients with tongue carcinoma:(-)20,(+)16,(++) 8,MVD was 39.18 per 200 ×field(SD=3.62).There was a positive correlation between them.②Between cervical lymph node metastasis group and non-metastasis groups ,VEGF and MVD and tumor size as well as clinical staging were significantly different .But in the statistics , VEGF and MVD played more important role .③Between the recurrence group and non-recurrence group ,VEGF and MVD were significantly different.Conclusion VEGF and MVD are closely related with infiltrative metastasis and recurrence in tongue squamous cell carcinoma .